Re: 请教蛋白保存问题# Biology - 生物学
M*e
1 楼
cystal of ice will form that can mechanically shear the protein.
the same as DNA. for storage of DNA, it is better to be stored
in buffer to avoid such mechanical shearing and hydrolysis,
if you concern about EDTA, you can use Tris-HCl (pH 8.0).
Here is some general guide for storage of purified proteins:
For short term storage (up to 24 h), most proteins can be kept
at 4C. For storage times longer than 24 h r at 4C, it may be
necessary to filter sterilize the protein preparation (through
a 0.22
the same as DNA. for storage of DNA, it is better to be stored
in buffer to avoid such mechanical shearing and hydrolysis,
if you concern about EDTA, you can use Tris-HCl (pH 8.0).
Here is some general guide for storage of purified proteins:
For short term storage (up to 24 h), most proteins can be kept
at 4C. For storage times longer than 24 h r at 4C, it may be
necessary to filter sterilize the protein preparation (through
a 0.22