Re: western blot问题# Biology - 生物学
M*e
1 楼
you should set constant VOLTAGE instead of CURRENT.
i use semidry from BioRad, and it works very nice for my
western. set to 15V, transfer @ RT for 1hr to 1.5hr. i never
think that all the protein could be transferred onto the
membrane. and thus to make a homogenous sandwitch is very
important, which means that you treat the gel, the membrane,
as well as the blotting paper very equally well.
think about this, it is the electric field that pushes the
charged molecules to move rather than the curr
i use semidry from BioRad, and it works very nice for my
western. set to 15V, transfer @ RT for 1hr to 1.5hr. i never
think that all the protein could be transferred onto the
membrane. and thus to make a homogenous sandwitch is very
important, which means that you treat the gel, the membrane,
as well as the blotting paper very equally well.
think about this, it is the electric field that pushes the
charged molecules to move rather than the curr