Re: transcription activator -> regulated genes# Biology - 生物学
s*e
1 楼
there are several ways you can think about, although techniquely,
they are quite difficult.
(1) If you know what is the transcription activator, there should
have a databank contain promoter sequences. (Sorry, I can't remember
the webste)
(2) You can use CHiP analysis to clone out DNA sequences that bind
to your protein. First crosslink, then sonicate, then immunoprecipitation,
then clone and sequence, then search database.
(3) As u need those genes regulated in the early 4hrs. Tranditional
over
they are quite difficult.
(1) If you know what is the transcription activator, there should
have a databank contain promoter sequences. (Sorry, I can't remember
the webste)
(2) You can use CHiP analysis to clone out DNA sequences that bind
to your protein. First crosslink, then sonicate, then immunoprecipitation,
then clone and sequence, then search database.
(3) As u need those genes regulated in the early 4hrs. Tranditional
over