Re: 有没有做4-piece ligation的?# Biology - 生物学
a*o
1 楼
Nothing special. Inserts ratio should be 1:1:1. Vector amount around ~50ng.
Inserts the more the better.
I use epicentre's fast-link ligase. I do three-way ligation routinely and
never failed once. Once for a while I do 4-way ligation, sometimes even with a
blunt end:)
stupid.
Inserts the more the better.
I use epicentre's fast-link ligase. I do three-way ligation routinely and
never failed once. Once for a while I do 4-way ligation, sometimes even with a
blunt end:)
stupid.