Re: Help! Lentiviral expression system# Biology - 生物学
w*t
1 楼
If things happened like that, why not design a control such as GFP insterted
into your vector, so it is easy to check the infection efficiency. It is not
surprised some cells survived after drug selection, there is why you need to
confirm the ectopic expression of your targer gene in stable cell line by WB
or other ways.