I have been trying to show protein interaction between a kinase and its predicted substrate by regular co- immunoprecipitation. but failed twice already. boss suggested crosslink co-IP again and again, very nicely though. I heard it is almost impossible to publish protein interaction data coming out from crosslink co-IP. every Daxia, Anybody see any published paper having crosslink co-IP data in it? If you do , please forward me the basic info ( journal and author or title). Thanks a lot. any ot
Have you ever sonicated your cell lysis before CO-IP?
m*k
20 楼
楼上的都几天拿到的?
z*a
21 楼
yes, I have.
h*n
22 楼
I suggest you not doing a sonication since it may kill some proteins. If your cell lysis is very sticky just vigorously pipette your samples up and down several times untill foaming.