Hi, folks: Any of you do primary mouse neuron culture? I like to know which stage one can easily derive primary neurons, the later the better for me... We have protocols for embryonic neurons but I'll like something later than that but aslo need to be healthy and can tolerate with some manipulation later on such as Cre-viral infection....
【在 n********k 的大作中提到】 : Hi, folks: : Any of you do primary mouse neuron culture? I like to know which stage : one can easily derive primary : neurons, the later the better for me... We have protocols for embryonic : neurons but I'll like something later : than that but aslo need to be healthy and can tolerate with some : manipulation later on such as Cre-viral : infection....
I use p1-3 usually. Occasionally old pups such as p20 can also be used but you might get only several thousands cells per ml and those cells might not grow very well. So the younger the better.
【在 n********k 的大作中提到】 : Hi, folks: : Any of you do primary mouse neuron culture? I like to know which stage : one can easily derive primary : neurons, the later the better for me... We have protocols for embryonic : neurons but I'll like something later : than that but aslo need to be healthy and can tolerate with some : manipulation later on such as Cre-viral : infection....
1) semaphore has two types: counting semaphore and binary semaphore 2) mutex is similar to binary semaphore but with one biggest difference "the principle of ownership" Mutex requires ownership, semaphore does not. That's it.