电生理外行请教一下LTP相关问题。 - 未名空间MITBBS历史存档

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电生理外行请教一下LTP相关问题。

电生理外行请教一下LTP相关问题。# Biology - 生物学

b*r2010-10-20 07:10

1 楼

现在已经收到faculty offer,延期一年上岗.期间在一个industry research center做
博后(research scientist). 本人目前在欧洲,请问申请何种visa比较合适?以下有两种
考虑
1:J1一年,然后到学校后再申请H1b，请问这一年中间是否能顺利完成waive转成H1b？J1期间申请
EB1，还是等到学校上岗后再EB1好？
2:H1b一年，到学校后再H1b。现在距离到岗时间还有4个月，是否来得及申请第一年的H1b？这种情
况，是否值得在第一年申请EB1？
谢谢

p*y2010-10-20 07:10

2 楼

1。飞机10：05到三藩，然后11：15起飞回国。不需要再取行李了吧？还需要在三藩重
新安检吗？
2。两段的boarding pass　是不是都在第一站的出发时候换？
3.I-94什么时候交？
谢谢了！！

f*42010-10-20 07:10

3 楼

现在做LTP一般是在活体内还是在脑片上。在活体内难吗？请教有没有经典的文献。谢
谢

s*u2010-10-20 07:10

4 楼

来得及申请第一年的H1b if you pay $1225 pp Fee.
值得在第一年申请EB1

r*l2010-10-20 07:10

5 楼

1. 不用取行李，不用过安检。
2. 应该是第一站都给你。
3. 三藩登机前交给agent。

【在 p******y 的大作中提到】

: 1。飞机10：05到三藩，然后11：15起飞回国。不需要再取行李了吧？还需要在三藩重
: 新安检吗？
: 2。两段的boarding pass　是不是都在第一站的出发时候换？
: 3.I-94什么时候交？
: 谢谢了！！

i*R2010-10-20 07:10

6 楼

我也比较外行。印象里LTP一般是在脑片上，很少能在活体上做细胞水平电生理的。虽
然德国有个组的确做到了直接在活动的老鼠脑部做电生理，但那技术是很难的。

a*y2010-10-20 07:10

7 楼

Why must pp? I thought faculty h1b is non-capped? Takes about three months
to get so that should be enough time?

【在 s**u 的大作中提到】

: 来得及申请第一年的H1b if you pay $1225 pp Fee.
: 值得在第一年申请EB1

w*02010-10-20 07:10

8 楼

1，联票肯定不需要取行李
2，如果不是同一家航空公司，比如ua转国航，在三藩需要重新办登机牌。
3，应该在三藩登机收，不过我有一次全程ua，在始发城市check in的时候就把我的i-
94收走啦。

f*42010-10-20 07:10

9 楼

谢谢回复
那活体上直接做的（就是说不是细胞水平的），意义大吗。
看这片文献http://www.sciencemag.org/cgi/data/309/5743/2033/DC1/1
Electrophysiology. Tc1 and wild-type littermate mice, aged 4-8 months, were
anaesthetized with an intraperitoneal injection of urethane (1.8 g/kg). LTP
was investigated in the dentate gyrus using a concentric bipolar stimulating
electrode positioned in the perforant path (AP-level with Lamda, ML-3 mm
from midline, DV-1.5 mm) and a glass micropipette recording electrode
positioned in the hilus of the ipsilateral dentate gyrus (AP-2 mm posterior
to bregma, ML-1.6 mm from midline, DV-1.5 mm, with minor variation judged by
shape of response). Test responses were evoked using monophasic stimuli (0.
033 Hz; 70-300 μA, 60 μs). Using a wide range of stimulus intensities
input-output curves were generated plotting the average of three sampled
responses for each intensity. Prior to assessment of LTP pairs of pulses (
inter-pulse intervals, 10- 1000 ms) were used to study paired-pulse
facilitation of EPSP and population spike. EPSP slope facilitation was
studied using a stimulus intensity sufficient to drive a pure EPSP response
of around 1 mV. Population spike facilitation was studied using a higher
stimulus intensity sufficient to evoke a spike of approximately 1 mV. Three
responses were also averaged for each interpulse interval. To study LTP a
baseline stimulus intensity was selected sufficient to drive a spike of
approximately 1 mV and an EPSP slope approximately 40% of maximum. 40
baseline recordings were made over 20 min prior to applying a tetanic
stimulation, consisting of 6 series of 6 trains of 6 stimuli at 400 Hz, 200
ms between trains, 20 s between series. During the tetanus pulse width was
doubled. 120 further evoked recordings were then made at baseline frequency
and intensity over the course of the following hour. The slope of the field
excitatory postsynaptic potential (fEPSP) was used as an index of LTP and
expressed as a percentage change relative to the mean response in the final
10 min before tetanus. Data are given as mean ± SEM.

【在 i***R 的大作中提到】

: 我也比较外行。印象里LTP一般是在脑片上，很少能在活体上做细胞水平电生理的。虽
: 然德国有个组的确做到了直接在活动的老鼠脑部做电生理，但那技术是很难的。

b*r2010-10-20 07:10

10 楼

再问个问题，如果是第一年H1b，是不是也要等到10月1号以后才能正式开始上班。这边
的合同到7月底，这样的话中间的gap可有什么办法？

【在 a**********y 的大作中提到】

: Why must pp? I thought faculty h1b is non-capped? Takes about three months
: to get so that should be enough time?

w*y2010-10-20 07:10

11 楼

意义大不大跟具体研究的问题有关吧。活体实验一般能把生理机制跟行为功能直接联系
起来，笼统的讲比脑片意义大。反之，如果不直接研究生理功能，就简单的做一些电生
理数据测量，即便实验难度大，意义也有限。

were
LTP
stimulating
posterior
by

【在 f***4 的大作中提到】

: 谢谢回复
: 那活体上直接做的（就是说不是细胞水平的），意义大吗。
: 看这片文献http://www.sciencemag.org/cgi/data/309/5743/2033/DC1/1
: Electrophysiology. Tc1 and wild-type littermate mice, aged 4-8 months, were
: anaesthetized with an intraperitoneal injection of urethane (1.8 g/kg). LTP
: was investigated in the dentate gyrus using a concentric bipolar stimulating
: electrode positioned in the perforant path (AP-level with Lamda, ML-3 mm
: from midline, DV-1.5 mm) and a glass micropipette recording electrode
: positioned in the hilus of the ipsilateral dentate gyrus (AP-2 mm posterior
: to bregma, ML-1.6 mm from midline, DV-1.5 mm, with minor variation judged by