关于in-situ探针设计中如何区分isoform# Biology - 生物学
D*D
1 楼
best Sci-fi u-tube movie!!! Don't miss it, update every Wednesday 12pm.
http://www.youtube.com/user/HplusDigitalSeries?feature=watch
http://www.youtube.com/user/HplusDigitalSeries?feature=watch
w*g
2 楼
兑现诺言, 今天买了乔总传.自己看接着看KINDLE把版的, 这本给娃看.
m*5
3 楼
我有个蛋白，已知有两个isoform,A form是全长，B form是trancated 的Aform
请问要如何设计探针tell them apart呢？它们的中间片段并没有区别
请问要如何设计探针tell them apart呢？它们的中间片段并没有区别
D*D
4 楼
and very interesting structure for a tube movie
Bravo Bryan Singer!!!
Bravo Bryan Singer!!!
r*y
5 楼
赞！想起了学习主席著作的照片，看这样子肯定听话不尿床
【在 w*****g 的大作中提到】
: 兑现诺言, 今天买了乔总传.自己看接着看KINDLE把版的, 这本给娃看.
【在 w*****g 的大作中提到】
: 兑现诺言, 今天买了乔总传.自己看接着看KINDLE把版的, 这本给娃看.
s*s
6 楼
没做过。不过我猜用LNA，PNA这类探针，一个设计在
truncation里面，一个设计跨在两边
【在 m******5 的大作中提到】
: 我有个蛋白，已知有两个isoform,A form是全长，B form是trancated 的Aform
: 请问要如何设计探针tell them apart呢？它们的中间片段并没有区别
truncation里面，一个设计跨在两边
【在 m******5 的大作中提到】
: 我有个蛋白，已知有两个isoform,A form是全长，B form是trancated 的Aform
: 请问要如何设计探针tell them apart呢？它们的中间片段并没有区别
h*k
7 楼
not bad
w*e
8 楼
没理解。用A有B没有的部分做antigen不行吗？
【在 m******5 的大作中提到】
: 我有个蛋白，已知有两个isoform,A form是全长，B form是trancated 的Aform
: 请问要如何设计探针tell them apart呢？它们的中间片段并没有区别
【在 m******5 的大作中提到】
: 我有个蛋白，已知有两个isoform,A form是全长，B form是trancated 的Aform
: 请问要如何设计探针tell them apart呢？它们的中间片段并没有区别
D*D
9 楼
yes, very intriguing plot, I am still trying to piece every information
together.
【在 h*****k 的大作中提到】
: not bad
together.
【在 h*****k 的大作中提到】
: not bad
t*o
10 楼
yeah you need to do that b/c they intentionally rip the story apart into a
jigsaw puzzle. more suspension but kinda unnecessary.
【在 D******D 的大作中提到】
: yes, very intriguing plot, I am still trying to piece every information
: together.
jigsaw puzzle. more suspension but kinda unnecessary.
【在 D******D 的大作中提到】
: yes, very intriguing plot, I am still trying to piece every information
: together.
D*D
11 楼
It is a matter of taste. I absolutely love it, so far it seemed some people
in SFO were 100% robots???
【在 t******o 的大作中提到】
: yeah you need to do that b/c they intentionally rip the story apart into a
: jigsaw puzzle. more suspension but kinda unnecessary.
in SFO were 100% robots???
【在 t******o 的大作中提到】
: yeah you need to do that b/c they intentionally rip the story apart into a
: jigsaw puzzle. more suspension but kinda unnecessary.
t*o
12 楼
no clue. so far
story 1: 7 years ago in Finland,a staged romance for a cyber crime cop,
probably the start of the technology that eventually destroyed the world
story 2: an Indian girl (very pretty I must say) testing out the technology
as a surrogate mother? a doctor sounds like a conspirator, a cut-throat
bitch scientist-businesswoman, a weak husband, possibly the start of how
something good turned ugly
story 3: airport where pretty much everyone died, a scientist who partially
caused the mayhem trying to save a few, some homeless punks without the chip
planed to take over the world.
story 4: 2 years later in intaly, how people suffered after getting kicked
back to stone age.
people
【在 D******D 的大作中提到】
: It is a matter of taste. I absolutely love it, so far it seemed some people
: in SFO were 100% robots???
story 1: 7 years ago in Finland,a staged romance for a cyber crime cop,
probably the start of the technology that eventually destroyed the world
story 2: an Indian girl (very pretty I must say) testing out the technology
as a surrogate mother? a doctor sounds like a conspirator, a cut-throat
bitch scientist-businesswoman, a weak husband, possibly the start of how
something good turned ugly
story 3: airport where pretty much everyone died, a scientist who partially
caused the mayhem trying to save a few, some homeless punks without the chip
planed to take over the world.
story 4: 2 years later in intaly, how people suffered after getting kicked
back to stone age.
people
【在 D******D 的大作中提到】
: It is a matter of taste. I absolutely love it, so far it seemed some people
: in SFO were 100% robots???
D*D
13 楼
The girl 7 years ago in Finland appeared in "2 years after in Italy"
and the three guys in SFO searching for the scientist who is trapped in the
parking lot seem to be 100% robots.
technology
partially
chip
【在 t******o 的大作中提到】
: no clue. so far
: story 1: 7 years ago in Finland,a staged romance for a cyber crime cop,
: probably the start of the technology that eventually destroyed the world
: story 2: an Indian girl (very pretty I must say) testing out the technology
: as a surrogate mother? a doctor sounds like a conspirator, a cut-throat
: bitch scientist-businesswoman, a weak husband, possibly the start of how
: something good turned ugly
: story 3: airport where pretty much everyone died, a scientist who partially
: caused the mayhem trying to save a few, some homeless punks without the chip
: planed to take over the world.
and the three guys in SFO searching for the scientist who is trapped in the
parking lot seem to be 100% robots.
technology
partially
chip
【在 t******o 的大作中提到】
: no clue. so far
: story 1: 7 years ago in Finland,a staged romance for a cyber crime cop,
: probably the start of the technology that eventually destroyed the world
: story 2: an Indian girl (very pretty I must say) testing out the technology
: as a surrogate mother? a doctor sounds like a conspirator, a cut-throat
: bitch scientist-businesswoman, a weak husband, possibly the start of how
: something good turned ugly
: story 3: airport where pretty much everyone died, a scientist who partially
: caused the mayhem trying to save a few, some homeless punks without the chip
: planed to take over the world.
t*o
14 楼
Yeah. Don't know how the the girl ended up on Italy. The 3 (or 4?) homeless
guys couldn't afford the technology and that's why they survived. My
understanding. But the scientist lied about not having wireless signal on
that floor. That may imply some of the survivors (the medical staff girl,
her mom, the wife, the son, a hoody guy) are of different species.
Let's see how long I can stay up with this format before getting bored. lol
the
【在 D******D 的大作中提到】
: The girl 7 years ago in Finland appeared in "2 years after in Italy"
: and the three guys in SFO searching for the scientist who is trapped in the
: parking lot seem to be 100% robots.
:
: technology
: partially
: chip
guys couldn't afford the technology and that's why they survived. My
understanding. But the scientist lied about not having wireless signal on
that floor. That may imply some of the survivors (the medical staff girl,
her mom, the wife, the son, a hoody guy) are of different species.
Let's see how long I can stay up with this format before getting bored. lol
the
【在 D******D 的大作中提到】
: The girl 7 years ago in Finland appeared in "2 years after in Italy"
: and the three guys in SFO searching for the scientist who is trapped in the
: parking lot seem to be 100% robots.
:
: technology
: partially
: chip
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