how to determine protein concentration that is bound to lipid membrane???# Biology - 生物学
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Does anyone know how to measure the concentration of protein that is bound
to lipid membrane? I can not measure it by UV/Vis because the phospholipid
greatly interferences the UV/Vis absorbance at very wide range.
I found a paper that used densitometry with a software (ImageQuant) to
determine the membrane bound protein concentration. Does anyone know how
reliable it would be. Many thanks!!!
to lipid membrane? I can not measure it by UV/Vis because the phospholipid
greatly interferences the UV/Vis absorbance at very wide range.
I found a paper that used densitometry with a software (ImageQuant) to
determine the membrane bound protein concentration. Does anyone know how
reliable it would be. Many thanks!!!
