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请教免疫组化/亚细胞结构染色的牛人

请教免疫组化/亚细胞结构染色的牛人# Biology - 生物学

l*g2011-04-09 07:04

1 楼

My son is over 2.5 yrs old and will start his preschool. We need to offer
him the breakfast at home. My question is should I offer him normal Chinese
breakfast or Milk only since the morning time is always so challenging.
Which one is more important? We want him to have enough food until his lunch
(BTW, he does has an early lunch scheudle at school at 11AM), but he also
needs to complete his morning meal as quickly as possible and head to school
:) He normally do milk much faster than the foo

g*t2011-04-09 07:04

2 楼

二十年前我就肯定确定以及一定ann有用
我的硕士论文是ann
Ann本身就是非线性滤波器
怎么会没用? 我说的是单层神经网。还不是deep learning.
Ann能拿来更好的控制温箱的温度你信不信。
为什么一般公司没人用？
因为懂的人太少了
一般公司知道什么是矩阵的condition number
什么是cholesky分解的总共才有几个啊...
讲究ann过于扯淡了
更重要的是tool chain,tech stack不行
所以应用ann的成本是很高的。
但是你查查军工...
咱们不扯什么智能不智能
投入这么大的软件ecosystem ，
能没用吗？起码能普及个多线性代数吧！
起码统计学习的一堆指标和技术术语会
进入很多产品说明书吧？到时候别人都会的，你听不懂...
也可以很愉快？
哭天喊地说搞AI都是大骗子的，不如换个角度想想。
不用你当骗子，还能享受骗子的成果，这种美好的事，有什么好悲愤交加的呢......

m*i2011-04-09 07:04

3 楼

我想确定一个受体在神经细胞的synaptic cleft(post-synaptic)里面的表达情况，
因为没做过相关实验，不知道目前用哪种技术。当然我想最后成像时ｃｏｎｆｏｃａｌ或者电镜应
该用得上。
假设试剂、抗体、设备都没问题，请做过相似实验的牛人指点一下哪种方法比较好，大
致怎么做。或者知道的给个ｒｅｆｅｒｅｎｃｅ就行。在此先行谢过！

m*k2011-04-09 07:04

4 楼

milk only is not a good idea.
having some balanced breakfast is very important.
some carbs, some protein. some fat.. and some fiber/milk and fruit.
cherrios, eggs, cheese. etc

Chinese
lunch
school

【在 l****g 的大作中提到】

: My son is over 2.5 yrs old and will start his preschool. We need to offer
: him the breakfast at home. My question is should I offer him normal Chinese
: breakfast or Milk only since the morning time is always so challenging.
: Which one is more important? We want him to have enough food until his lunch
: (BTW, he does has an early lunch scheudle at school at 11AM), but he also
: needs to complete his morning meal as quickly as possible and head to school
: :) He normally do milk much faster than the foo

x*u2011-04-09 07:04

5 楼

用决策树更好吧

【在 g****t 的大作中提到】

: 二十年前我就肯定确定以及一定ann有用
: 我的硕士论文是ann
: Ann本身就是非线性滤波器
: 怎么会没用? 我说的是单层神经网。还不是deep learning.
: Ann能拿来更好的控制温箱的温度你信不信。
: 为什么一般公司没人用？
: 因为懂的人太少了
: 一般公司知道什么是矩阵的condition number
: 什么是cholesky分解的总共才有几个啊...
: 讲究ann过于扯淡了

i*l2011-04-09 07:04

6 楼

我会先做ｃｏｎｆｏｃａｌ immuno fluorescence microscopy
再想看细微的亚细胞结构，或者不同刺激下的Translocation，免疫电镜可能会更好

ｌ或者电镜应

【在 m***i 的大作中提到】

: 我想确定一个受体在神经细胞的synaptic cleft(post-synaptic)里面的表达情况，
: 因为没做过相关实验，不知道目前用哪种技术。当然我想最后成像时ｃｏｎｆｏｃａｌ或者电镜应
: 该用得上。
: 假设试剂、抗体、设备都没问题，请做过相似实验的牛人指点一下哪种方法比较好，大
: 致怎么做。或者知道的给个ｒｅｆｅｒｅｎｃｅ就行。在此先行谢过！

x*t2011-04-09 07:04

7 楼

agree. need to eat breakfast. it is always challenging to feed kids in the
morning, but you just have to do it for his health/growth reason.

【在 m**k 的大作中提到】

: milk only is not a good idea.
: having some balanced breakfast is very important.
: some carbs, some protein. some fat.. and some fiber/milk and fruit.
: cherrios, eggs, cheese. etc
:
: Chinese
: lunch
: school

g*t2011-04-09 07:04

8 楼

哪个便宜，宣传效果好，就用哪个呗

: 用决策树更好吧

【在 x****u 的大作中提到】

: 用决策树更好吧

l*g2011-04-09 07:04

9 楼

Thanks for the advice!!!

l*g2011-04-09 07:04

10 楼

Thanks for the advice!!!