m*u
2 楼
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troyli (安逸公子) 于 (Mon Aug 3 19:04:46 2009, 美东) 提到:
公开歧视某个族裔总是不对要被讨伐地。。不过脑袋里的偏见和歧视是断不了地。。
就说今天出来顶劳模的几个ID, 我敢说他们买房子的时候第一件事儿就是远离黑裔和墨
裔聚集的地区。。因为学区，治安等等因素这个是肯定地。与其说他们是虚伪还不如说
是在这个现实社会生活的一种无奈。。
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SugarComa (One donut a day, keep 6pac away) 于 (Mon Aug 3 19:16:11 2009, 美东) 提到:
科学研究发现：女子每天喝咖啡超过三杯， 乳房会变小.
最好灭绝地球上所有的咖啡。
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troyli (安逸公子) 于 (Mon Aug 3 19:16:58 2009, 美东) 提到:
what?
troyli (安逸公子) 于 (Mon Aug 3 19:04:46 2009, 美东) 提到:
公开歧视某个族裔总是不对要被讨伐地。。不过脑袋里的偏见和歧视是断不了地。。
就说今天出来顶劳模的几个ID, 我敢说他们买房子的时候第一件事儿就是远离黑裔和墨
裔聚集的地区。。因为学区，治安等等因素这个是肯定地。与其说他们是虚伪还不如说
是在这个现实社会生活的一种无奈。。
☆─────────────────────────────────────☆
SugarComa (One donut a day, keep 6pac away) 于 (Mon Aug 3 19:16:11 2009, 美东) 提到:
科学研究发现：女子每天喝咖啡超过三杯， 乳房会变小.
最好灭绝地球上所有的咖啡。
☆─────────────────────────────────────☆
troyli (安逸公子) 于 (Mon Aug 3 19:16:58 2009, 美东) 提到:
what?
s*y
3 楼
A WOMAN SHOULD HAVE
...one old love
she can imagine
going back to...
and one who reminds
her how far she has come...
A WOMAN SHOULD HAVE
...enough money within her
control to move out and
rent a place of her own
even if she never wants
to or needs to...
A WOMAN SHOULD HAVE
.. something perfect to wear if
the employer or date of her dreams
wants to see her in an hour...
A WOMAN SHOULD HAVE
.. a youth she's content
to leave behind...
A WOMAN SHOULD HAVE
...a past juicy enough that
she's looking forward to
retelling it in her old age....
A WOMAN SHOULD HAVE
...a set of screwdrivers, a
cordless drill, and a black
lace bra...
A WOMAN SHOULD HAVE
..one friend who always makes
her laugh ... and one who lets
her cry...
A WOMAN SHOULD HAVE
...a good piece of furniture
not previously owned by anyone
else in her family...
A WOMAN SHOULD HAVE
...eight matching plates, wine
glasses with stems, and a recipe
for a meal that will make her
guests feel honored.
A WOMAN SHOULD HAVE
...a feeling of control over
her destiny...
EVERY WOMAN SHOULD KNOW...
....how to fall in love without
losing herself...
EVERY WOMAN SHOULD KNOW...
..how to quit a job,
break up with a lover,
and confront a friend
without ruining the friendship...
EVERY WOMAN SHOULD KNOW...
...when to try harder ... and
when to walk away...
EVERY WOMAN SHOULD KNOW...
....that she can't change the
length of her calves, the width
of her hips, or the nature of her
parents...
EVERY WOMAN SHOULD KNOW...
...that her childhood may not
have been perfect...but its
over...
EVERY WOMAN SHOULD KNOW...
...what she would and wouldn't
do for love or more...
EVERY WOMAN SHOULD KNOW...
..how to live alone... even if
she doesn't like it...
EVERY WOMAN SHOULD KNOW...
...whom she can trust,
whom she can't,
and why she shouldn't
take it personally...
EVERY WOMAN SHOULD KNOW...
...where to go...
be it to her best friend's kitchen
table...
or a charming inn in the woods...
when her soul needs soothing...
EVERY WOMAN SHOULD KNOW...
.. what she can and can't
accomplish in a day...
a month...and a year...
http://holliesquotes.com/life/awomanshould.htm
...one old love
she can imagine
going back to...
and one who reminds
her how far she has come...
A WOMAN SHOULD HAVE
...enough money within her
control to move out and
rent a place of her own
even if she never wants
to or needs to...
A WOMAN SHOULD HAVE
.. something perfect to wear if
the employer or date of her dreams
wants to see her in an hour...
A WOMAN SHOULD HAVE
.. a youth she's content
to leave behind...
A WOMAN SHOULD HAVE
...a past juicy enough that
she's looking forward to
retelling it in her old age....
A WOMAN SHOULD HAVE
...a set of screwdrivers, a
cordless drill, and a black
lace bra...
A WOMAN SHOULD HAVE
..one friend who always makes
her laugh ... and one who lets
her cry...
A WOMAN SHOULD HAVE
...a good piece of furniture
not previously owned by anyone
else in her family...
A WOMAN SHOULD HAVE
...eight matching plates, wine
glasses with stems, and a recipe
for a meal that will make her
guests feel honored.
A WOMAN SHOULD HAVE
...a feeling of control over
her destiny...
EVERY WOMAN SHOULD KNOW...
....how to fall in love without
losing herself...
EVERY WOMAN SHOULD KNOW...
..how to quit a job,
break up with a lover,
and confront a friend
without ruining the friendship...
EVERY WOMAN SHOULD KNOW...
...when to try harder ... and
when to walk away...
EVERY WOMAN SHOULD KNOW...
....that she can't change the
length of her calves, the width
of her hips, or the nature of her
parents...
EVERY WOMAN SHOULD KNOW...
...that her childhood may not
have been perfect...but its
over...
EVERY WOMAN SHOULD KNOW...
...what she would and wouldn't
do for love or more...
EVERY WOMAN SHOULD KNOW...
..how to live alone... even if
she doesn't like it...
EVERY WOMAN SHOULD KNOW...
...whom she can trust,
whom she can't,
and why she shouldn't
take it personally...
EVERY WOMAN SHOULD KNOW...
...where to go...
be it to her best friend's kitchen
table...
or a charming inn in the woods...
when her soul needs soothing...
EVERY WOMAN SHOULD KNOW...
.. what she can and can't
accomplish in a day...
a month...and a year...
http://holliesquotes.com/life/awomanshould.htm
s*s
4 楼
想请教一下。我打算按某些gene的expression来sort细胞，
觉得这个MB是一个不错的选择。但是不知道这个是一个比较
容易的tech，像RNAi一样只要问公司买就行了，而且多半就
是work的；还是不容易work，设计很复杂?
另外，有没有推荐的公司？
觉得这个MB是一个不错的选择。但是不知道这个是一个比较
容易的tech，像RNAi一样只要问公司买就行了，而且多半就
是work的；还是不容易work，设计很复杂?
另外，有没有推荐的公司？
s*s
6 楼
补充一下，我说的是living cells里面的，不是taqman那种realtime probe
【在 s******s 的大作中提到】
: 想请教一下。我打算按某些gene的expression来sort细胞，
: 觉得这个MB是一个不错的选择。但是不知道这个是一个比较
: 容易的tech，像RNAi一样只要问公司买就行了，而且多半就
: 是work的；还是不容易work，设计很复杂?
: 另外，有没有推荐的公司？
【在 s******s 的大作中提到】
: 想请教一下。我打算按某些gene的expression来sort细胞，
: 觉得这个MB是一个不错的选择。但是不知道这个是一个比较
: 容易的tech，像RNAi一样只要问公司买就行了，而且多半就
: 是work的；还是不容易work，设计很复杂?
: 另外，有没有推荐的公司？
i*n
7 楼
THX
【在 f********i 的大作中提到】
: http://bankatwork.bankofamerica.com/?cm_mmc=DEP-GroupBanking-_-
d*m
8 楼
我靠，太亲切了，这年头竟然还有人用molecular beacons做imaging。
去那个发明MB的印度人的公司看看啊，我记得是提供全部设计的。
去那个发明MB的印度人的公司看看啊，我记得是提供全部设计的。
s*s
9 楼
你用过？哪个公司啊？能不能给个link?
我只是想根据基因表达sort一些living cell，这种不是surface marker的
不能stain, promoter不清楚的做reporter麻烦而且慢，用MB是不是适用？
【在 d********m 的大作中提到】
: 我靠，太亲切了，这年头竟然还有人用molecular beacons做imaging。
: 去那个发明MB的印度人的公司看看啊，我记得是提供全部设计的。
我只是想根据基因表达sort一些living cell，这种不是surface marker的
不能stain, promoter不清楚的做reporter麻烦而且慢，用MB是不是适用？
【在 d********m 的大作中提到】
: 我靠，太亲切了，这年头竟然还有人用molecular beacons做imaging。
: 去那个发明MB的印度人的公司看看啊，我记得是提供全部设计的。
d*m
10 楼
我以前在国内带我做实验的那哥们就是用molecular beacon tracking virus mRNA in
living cells。看了不少文献，没自己设计过，因为很贵不能拿来练手。不过听他说起
来应该挺容易的。而且国内也没有现成的做MB的公司，他都是自己设计的stem-loop结
构，文章最后还发在nucleic acid research了，应该挺靠谱的。设计参照这个网站http://www.molecular-beacons.com/#
living cells。看了不少文献，没自己设计过，因为很贵不能拿来练手。不过听他说起
来应该挺容易的。而且国内也没有现成的做MB的公司，他都是自己设计的stem-loop结
构，文章最后还发在nucleic acid research了，应该挺靠谱的。设计参照这个网站http://www.molecular-beacons.com/#
s*s
11 楼
谢谢。我也看到的是这个网站，不过人家都是edu的institute, 我研究了半天
也没搞清楚是研究所合作呢还是花钱公司买
in
【在 d********m 的大作中提到】
: 我以前在国内带我做实验的那哥们就是用molecular beacon tracking virus mRNA in
: living cells。看了不少文献，没自己设计过，因为很贵不能拿来练手。不过听他说起
: 来应该挺容易的。而且国内也没有现成的做MB的公司，他都是自己设计的stem-loop结
: 构，文章最后还发在nucleic acid research了，应该挺靠谱的。设计参照这个网站http://www.molecular-beacons.com/#
也没搞清楚是研究所合作呢还是花钱公司买
in
【在 d********m 的大作中提到】
: 我以前在国内带我做实验的那哥们就是用molecular beacon tracking virus mRNA in
: living cells。看了不少文献，没自己设计过，因为很贵不能拿来练手。不过听他说起
: 来应该挺容易的。而且国内也没有现成的做MB的公司，他都是自己设计的stem-loop结
: 构，文章最后还发在nucleic acid research了，应该挺靠谱的。设计参照这个网站http://www.molecular-beacons.com/#
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