关于DNA聚合酶的问题 - 未名空间MITBBS历史存档

国际科技财经博客移民网络热点娱乐民生时事公众号

Redian新闻

>未名空间

>Biology - 生物学

关于DNA聚合酶的问题

关于DNA聚合酶的问题# Biology - 生物学

T*n2012-04-16 07:04

1 楼

跟原来想的十分钟root掉差不多
Kindle Fire gets rooted with 'one click,' paves the way for Amazonian
adventures
http://www.engadget.com/2011/11/16/kindle-fire-gets-rooted-with
xda上的详细流程
http://forum.xda-developers.com/showthread.php?t=1348830

f*g2012-04-16 07:04

2 楼

DNA聚合酶每天都在不停的合成DNA，包括高等动物，低等动物，原核，真核。但是在不
停的合成中会产生突变。
我的问题是哪种DNA聚合酶的突变率最低?
到目前为止，to my knowledge, 能够买到的DNA聚合酶中，phusion的突变率是最低的
。有没有可能人的DNA聚合酶的突变率比phusion更低呢?
thank you

a*o2012-04-16 07:04

3 楼

199够买好多其它强大得多的7吋板子了啊。

【在 T****n 的大作中提到】

: 跟原来想的十分钟root掉差不多
: Kindle Fire gets rooted with 'one click,' paves the way for Amazonian
: adventures
: http://www.engadget.com/2011/11/16/kindle-fire-gets-rooted-with
: xda上的详细流程
: http://forum.xda-developers.com/showthread.php?t=1348830

f*g2012-04-16 07:04

4 楼

ding

T*n2012-04-16 07:04

5 楼

kf的优势还是amazon的内容吧
root不过是撒点盐

【在 a*o 的大作中提到】

: 199够买好多其它强大得多的7吋板子了啊。

f*g2012-04-16 07:04

6 楼

ding

h*e2012-04-16 07:04

7 楼

for instance?

【在 a*o 的大作中提到】

: 199够买好多其它强大得多的7吋板子了啊。

f*g2012-04-16 07:04

8 楼

这个领域的xdjm给点文献也成啊

t*t2012-04-16 07:04

9 楼

vizio tablet

【在 h*e 的大作中提到】

: for instance?

f*g2012-04-16 07:04

10 楼

DNA聚合酶的核心doamin是什么?

S*h2012-04-16 07:04

11 楼

Vizio 8" Android 2.3 Tablet. This one comes with camera and GPS. $189.
http://www.costco.com/Browse/Product.aspx?prodid=11663717&whse=

f*g2012-04-16 07:04

12 楼

ding

t*y2012-04-16 07:04

13 楼

It is also reported to be slow.

【在 S*******h 的大作中提到】

: Vizio 8" Android 2.3 Tablet. This one comes with camera and GPS. $189.
: http://www.costco.com/Browse/Product.aspx?prodid=11663717&whse=

K*42012-04-16 07:04

14 楼

Well, I can not give you an answer to your question like 哪种DNA聚合酶的突变
率最低?
But from my friend's work, I can tell you that for DNA replication, many polymerases are involved, but only one is the primary enzyme. In the case of archaea and eukaryotes, this is Pol II (or better known as Family B DNA polymerase) and in bacteria, this is Pol III. Also note that Pol II and Pol III belong to distinct folds.
In nature, organisms dont need to maintain very reliable polymerases since allowing mutation during DNA synthesis is a need for the evolution, just like what viruses do. Thus I guess those commercial enzymes like phusion may have 突变率最低 since they have been optimized.

【在 f******g 的大作中提到】

: DNA聚合酶每天都在不停的合成DNA，包括高等动物，低等动物，原核，真核。但是在不
: 停的合成中会产生突变。
: 我的问题是哪种DNA聚合酶的突变率最低?
: 到目前为止，to my knowledge, 能够买到的DNA聚合酶中，phusion的突变率是最低的
: 。有没有可能人的DNA聚合酶的突变率比phusion更低呢?
: thank you

o*t2012-04-16 07:04

15 楼

这个东东的性价比究竟高在哪里？

【在 T****n 的大作中提到】

f*g2012-04-16 07:04

16 楼

thank you so much.

polymerases are involved, but only one is the primary enzyme. In the case of
archaea and eukaryotes, this is Pol II (or better known as Family B DNA
polymerase) and in bacteria, this is Pol III. Also note that Pol II and Pol
III belong to distinct folds.
allowing mutation during DNA synthesis is a need for the evolution, just
like what viruses do. Thus I guess those commercial enzymes like phusion may
have 突变率最低 since they have been optimized.

【在 K**4 的大作中提到】

: Well, I can not give you an answer to your question like 哪种DNA聚合酶的突变
: 率最低?
: But from my friend's work, I can tell you that for DNA replication, many polymerases are involved, but only one is the primary enzyme. In the case of archaea and eukaryotes, this is Pol II (or better known as Family B DNA polymerase) and in bacteria, this is Pol III. Also note that Pol II and Pol III belong to distinct folds.
: In nature, organisms dont need to maintain very reliable polymerases since allowing mutation during DNA synthesis is a need for the evolution, just like what viruses do. Thus I guess those commercial enzymes like phusion may have 突变率最低 since they have been optimized.