m*n
2 楼
快沉了
自己顶下
我就是用lentivirus包装的方法
然后感染，感觉work
不过没听说怎么做的，除了在版好像见过一次
就去试了下
【在 m*******n 的大作中提到】
: 试了下，western可以看到条带
: 但是没看到文献报道
: 跑过来请达人确认下
: 多谢了
自己顶下
我就是用lentivirus包装的方法
然后感染，感觉work
不过没听说怎么做的，除了在版好像见过一次
就去试了下
【在 m*******n 的大作中提到】
: 试了下，western可以看到条带
: 但是没看到文献报道
: 跑过来请达人确认下
: 多谢了
l*u
3 楼
不同病毒包装的质粒用的是不一样的
通常，retro的不能用于lenti
但是，没有试验过
通常，retro的不能用于lenti
但是，没有试验过
s*y
4 楼
很久很久以前我问过这个问题，当时的回答是：
能包进去，但是效率不会高。
【在 m*******n 的大作中提到】
: 试了下，western可以看到条带
: 但是没看到文献报道
: 跑过来请达人确认下
: 多谢了
能包进去，但是效率不会高。
【在 m*******n 的大作中提到】
: 试了下，western可以看到条带
: 但是没看到文献报道
: 跑过来请达人确认下
: 多谢了
m*n
5 楼
western跟pLM letivirus的带我gene的vector比
感觉两个的条带差不多呢
好奇怪
【在 s******y 的大作中提到】
: 很久很久以前我问过这个问题，当时的回答是：
: 能包进去，但是效率不会高。
感觉两个的条带差不多呢
好奇怪
【在 s******y 的大作中提到】
: 很久很久以前我问过这个问题，当时的回答是：
: 能包进去，但是效率不会高。
n*k
6 楼
I doubt you have done the readings...In short, yes and no...For some, the
lenti packging can be used for Retro(pbabe). So for dividing cells, doesn't
matter much for the infection/integration; However, for non-dividing cells,
presumably no big difference with infection but a huge difference with
integration step(nuclear entry step--which is essentially the key difference
between the two)...So essentially western doesn't tell you much here...
unless they stablize.
BTW, contrast to the common belief, retroviral can infect non-dividing cells
for sure but in general at much lower efficiency but with high titer virus,
one still get tons...As a field, this is something (intentionally?)
unspoken but really it could potentially be a huge caveat for certain
experimentations... So always keep being critical and don't easily believe
into something before careful/thorough examinations...
【在 m*******n 的大作中提到】
: 试了下，western可以看到条带
: 但是没看到文献报道
: 跑过来请达人确认下
: 多谢了
lenti packging can be used for Retro(pbabe). So for dividing cells, doesn't
matter much for the infection/integration; However, for non-dividing cells,
presumably no big difference with infection but a huge difference with
integration step(nuclear entry step--which is essentially the key difference
between the two)...So essentially western doesn't tell you much here...
unless they stablize.
BTW, contrast to the common belief, retroviral can infect non-dividing cells
for sure but in general at much lower efficiency but with high titer virus,
one still get tons...As a field, this is something (intentionally?)
unspoken but really it could potentially be a huge caveat for certain
experimentations... So always keep being critical and don't easily believe
into something before careful/thorough examinations...
【在 m*******n 的大作中提到】
: 试了下，western可以看到条带
: 但是没看到文献报道
: 跑过来请达人确认下
: 多谢了
m*n
7 楼
谢谢回复。我是在MCF7细胞里面做的
下次做个IF看看infeciton的效率
t
,
difference
cells
virus,
【在 n********k 的大作中提到】
: I doubt you have done the readings...In short, yes and no...For some, the
: lenti packging can be used for Retro(pbabe). So for dividing cells, doesn't
: matter much for the infection/integration; However, for non-dividing cells,
: presumably no big difference with infection but a huge difference with
: integration step(nuclear entry step--which is essentially the key difference
: between the two)...So essentially western doesn't tell you much here...
: unless they stablize.
: BTW, contrast to the common belief, retroviral can infect non-dividing cells
: for sure but in general at much lower efficiency but with high titer virus,
: one still get tons...As a field, this is something (intentionally?)
下次做个IF看看infeciton的效率
t
,
difference
cells
virus,
【在 n********k 的大作中提到】
: I doubt you have done the readings...In short, yes and no...For some, the
: lenti packging can be used for Retro(pbabe). So for dividing cells, doesn't
: matter much for the infection/integration; However, for non-dividing cells,
: presumably no big difference with infection but a huge difference with
: integration step(nuclear entry step--which is essentially the key difference
: between the two)...So essentially western doesn't tell you much here...
: unless they stablize.
: BTW, contrast to the common belief, retroviral can infect non-dividing cells
: for sure but in general at much lower efficiency but with high titer virus,
: one still get tons...As a field, this is something (intentionally?)
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