If flag-IL-17C could bind to surface IL-17RA or RE, after binding by surface staining of anti-flag-dye, there should be signal in flow cytometry analysis compared with without IL-17C control. There is a shift, although not strong in histogram.
1. in 293 cells 1.1 not expressing anything 1.2 express GFP 1.3 express IL17-RA 1.4 express IL17-RE 2. Treat IL17C on cells of "1". 2.1 Treated IL17C-->Bold Line. 2.2 Not treated with IL17C -->Shaded area 3. Detect IL17C by FLAG antibody in IL17C treated or not treated cells in "1" 4. If IL17C binds to cells in 1, you'll see stronger signal (bold line) than non-treated control (shaded area). 5. Because IL17C binds to IL17-RA and IL17-RE, you can see the bold lines shifting right in cells expressing IL17-RA and IL17-RE because more IL17C was retained on these cells than on 293 expressing nothing or GFP. 请问包子能干啥?
the solid line compared with the gray area in histogram solid line is with flag-IL17C, gray area means without. the two histograms in the lower row are experimental ones, another two in upper are control.
【在 I***a 的大作中提到】 : 这个shift是代表了什么?
i*y
18 楼
谢谢了,我都四十多了
【在 l*******r 的大作中提到】 : 大时代 挺轰动的 : 可能你年纪小,理解不了
J*s
19 楼
then I will get all the 5 baozi,lol
"1" than
【在 r****r 的大作中提到】 : 1. in 293 cells : 1.1 not expressing anything : 1.2 express GFP : 1.3 express IL17-RA : 1.4 express IL17-RE : 2. Treat IL17C on cells of "1". : 2.1 Treated IL17C-->Bold Line. : 2.2 Not treated with IL17C -->Shaded area : 3. Detect IL17C by FLAG antibody in IL17C treated or not treated cells in "1" : 4. If IL17C binds to cells in 1, you'll see stronger signal (bold line) than
I*a
20 楼
好, 2楼5个 4楼3个
J*s
21 楼
thanks :)
【在 I***a 的大作中提到】 : 好, : 2楼5个 : 4楼3个
I*a
22 楼
mitbbs的伪币 老型的美元,
【在 r****r 的大作中提到】 : 1. in 293 cells : 1.1 not expressing anything : 1.2 express GFP : 1.3 express IL17-RA : 1.4 express IL17-RE : 2. Treat IL17C on cells of "1". : 2.1 Treated IL17C-->Bold Line. : 2.2 Not treated with IL17C -->Shaded area : 3. Detect IL17C by FLAG antibody in IL17C treated or not treated cells in "1" : 4. If IL17C binds to cells in 1, you'll see stronger signal (bold line) than
r*r
23 楼
nice. thanks.
l*i
24 楼
难道这么小的差异也算?
"1" than
【在 r****r 的大作中提到】 : 1. in 293 cells : 1.1 not expressing anything : 1.2 express GFP : 1.3 express IL17-RA : 1.4 express IL17-RE : 2. Treat IL17C on cells of "1". : 2.1 Treated IL17C-->Bold Line. : 2.2 Not treated with IL17C -->Shaded area : 3. Detect IL17C by FLAG antibody in IL17C treated or not treated cells in "1" : 4. If IL17C binds to cells in 1, you'll see stronger signal (bold line) than
r*r
25 楼
well, i am not the reviewer. maybe they have more experiments to prove the interaction.