问个问题shRNA knockdown cell lines# Biology - 生物学
l*e
1 楼
I will have a onsite next Wendesday. It took me so long time to get this one
. Wish I can get an offer.
Pl bless me. Thnaks!
. Wish I can get an offer.
Pl bless me. Thnaks!
y*g
2 楼
请教大家几个问题
1、 选puromycin浓度的时候，需要提前做个proliferation 的试验来确定dose，大家
treat cells几天，是选80%抑制的dose么？
2、 作colony formation assay的时候，因为做的时间比较长，等的过程中，溶液中重
要加药物筛选么？
谢谢大家！！
1、 选puromycin浓度的时候，需要提前做个proliferation 的试验来确定dose，大家
treat cells几天，是选80%抑制的dose么？
2、 作colony formation assay的时候，因为做的时间比较长，等的过程中，溶液中重
要加药物筛选么？
谢谢大家！！
l*d
3 楼
BLESS!
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
j*n
4 楼
1、puromycin作用时间有时候会比较慢，视细胞株而异，建议做1个礼拜左右观察。建
议使用100%抑制的最低浓度，否则很可能混入假阳性克隆。
2、我一般3-5天换一次液。
议使用100%抑制的最低浓度，否则很可能混入假阳性克隆。
2、我一般3-5天换一次液。
w*s
5 楼
bless
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
c*h
6 楼
bless~
G*0
7 楼
Bless~
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
s*g
8 楼
bless
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
P*e
9 楼
bless~
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
t*y
10 楼
bless!
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
d*e
11 楼
bless
p*n
12 楼
Bless~
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
a*9
13 楼
belss
s*j
14 楼
big bless
a*r
15 楼
big big bless！
s*n
16 楼
one
【在 l*******e 的大作中提到】
: I will have a onsite next Wendesday. It took me so long time to get this one
: . Wish I can get an offer.
: Pl bless me. Thnaks!
H*f
17 楼
bless...
z*s
18 楼
Bless!
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