70kD band might be DnaK it's very normal to copurify the molecular chaperone when you try to purify those overexpressed fusion proteins to remove DnaK, you can add 5 mM ATP and 5 mM Mg2+ into lysate and incubate for 20 min at room temperature prior to purification (i.e., before loading onto the affinity column)
【在 b******n 的大作中提到】 : 70kD band might be DnaK : it's very normal to copurify the molecular chaperone when you try to purify : those overexpressed fusion proteins : to remove DnaK, you can add 5 mM ATP and 5 mM Mg2+ into lysate and incubate : for 20 min at room temperature prior to purification (i.e., before loading : onto the affinity column)
b*u
8 楼
一句都没问,偶的CASe [发表自未名空间手机版 - m.mitbbs.com]
p*u
9 楼
请问你用的是combo card吗?是不用进"小黑屋"了吗?谢谢
一句都没问,偶的CASe[发表自未名空间手机版 - m.mitbbs.com] ★ Sent from iPhone App: iReader Mitbbs Lite 7.36