they want to see your RAW data file, this file can tell you lots of information such as CID, fragmentation, signal-noise, signal intensities, etc. I would use XCalibur to make series of figures to show the CID, signal- noise ratio and intensities.
【在 G***G 的大作中提到】 : 在蛋质谱分析中,什么图是 : "higher resolution views of the primary proteomic data"? : 请指出这是要求补充什么图? : 1个包子,小小谢意。
K*S
7 楼
TIC is meaningless. You can probably ignore your 1st figure. 2nd figure (sounds like MS1), you need to zoom in the area where you detect the intact peptides, for high resolution data, you need to show all the isotopic peaks and at least 4 decimals of m/z, such as 1000.0000 (assuming you use high resolution instrument) 3rd figure you mentioned sounds like MS2 spectra, did you label the fragments?
【在 K******S 的大作中提到】 : TIC is meaningless. You can probably ignore your 1st figure. : 2nd figure (sounds like MS1), you need to zoom in the area where you detect : the intact peptides, for high resolution data, you need to show all the : isotopic peaks and at least 4 decimals of m/z, such as 1000.0000 (assuming : you use high resolution instrument) : 3rd figure you mentioned sounds like MS2 spectra, did you label the : fragments?