De novo assembly by Trinity http://trinityrnaseq.sourceforge.net/ References: a, PMID 23331995 BMC Genomics. 14:41. (2013). De novo transcriptome characterization of Vitis vinifera cv. Corvina unveils varietal diversity. http://www.ncbi.nlm.nih.gov/pubmed/23331995 or PMID 21572440 Nat Biotechnol. 29:644-52. (2011). Full-length transcriptome assembly from RNA-Seq data without a reference genome. http://www.ncbi.nlm.nih.gov/pubmed/21572440 or PMID 22965124 Nucleic Acids Res. 40:10084-97. (2012). A comprehensive comparison of RNA-Seq-based transcriptome analysis from reads to differential gene expression and cross-comparison with microarrays: a case study in Saccharomyces cerevisiae. http://www.ncbi.nlm.nih.gov/pubmed/22965124 original post from mitbbs older post: http://www.mitbbs.com/article_t/Biology/31569333.html 4th or 5th floor 发信人: cellcreator (cellcreator), 信区: Biology 标 题: Re: 简单介绍 Bioinformatics Tools for NGS 分析 发信站: BBS 未名空间站 (Mon Sep 12 02:24:54 2011, 美东) 我们领域做过的牛人也用过Trinity。听有经验的人都说de novo耗内存,Trinity说需 要1G RAM per 1M ~76 base Illumina paired reads。还好我做的transcriptome大概 12M 左右的reads,现在在准备装一个小型的工作站,先用16G 内存试一试看够不够。 有机会也去试试BGI的,BGI现在连着几期都在Nature上做广告了,知名度也在提升啊。 希望他家的东西也不错。^_^
'dChip' 的对应 R version 不能对应WIN7 悲剧鸟 if from next 2014 May WINXP 悲剧鸟 2 from microsoft suspended WINXP back up service then Byebye 'dChip' ? explain: 'dChip' should run in R code mode but but > Install R. This interface does not work with the latest R. Please install R 2.3.1 or R 1.6.2 instead. At the “Select Components” dialog you may only select “main files”. Start R through “Start/Programs/R” to confirm R is installed properly; later on dChip will automatically start R. For R-COM to run properly, you may need to restart the computer after installing R to add registry key of R path to Windows. https://sites.google.com/site/dchipsoft/introduction-to-dchip/interface-with -the-r-software and R 1.6.2 for Windows This directory contains a binary distribution of R-1.6.2 to run on Windows 95, 98, ME, NT4.0, 2000 and XP on Intel/clone chips. http://cran.us.r-project.org/bin/windows/base/old/1.6.2/ even alternatively R-2.3.1 for Windows This directory contains a binary distribution of R-2.3.1 to run on Windows 95, 98, ME, NT4.0, 2000 and XP on Intel/clone chips. http://cran.us.r-project.org/bin/windows/base/old/2.3.1/ References https://sites.google.com/site/dchipsoft/downloading-dchip-software http://www.hsph.harvard.edu/cli/complab/dchip/ http://www.stanford.edu/group/wonglab/software.html Summary: 'dChip' updated version from Harvard Cheng Li lab which can be interfaced with R either can run in WIN7 or WIN8 unless 'dChip' will be excluded from 2015Y on WINDOWS OS.
【在 f******g 的大作中提到】 : 不知道还有没有人在用,但是dchip一直在更新。
l*1
29 楼
Bedutils NGSUtils is a suite of software tools for working with next-generation sequencing datasets. Staring in 2009, we (Liu Lab @ Indiana University School of Medicine) starting working with next-generation sequencing data. We initially started doing custom coding for each project in a one-off manner. It quickly became apparent that this was an inefficient manner to work, so we started assembling smaller utilities that could be adapted into larger, more complicated, workflows. We have used them for Illumia, SOLiD and 454 sequencing data. We have used them for DNA and RNA resequcing, ChIP- Seq, CLIP-Seq, and targeted resequencing (Agilent exome capture and PCR targeting). These tools are also used heavily in our in-house DNA and RNA mapping pipelines. These tools have of great use within our lab group, and so we are happy to make them available to the greater community. NGSUtils is made up of 50+ programs, mainly written in Python. These are separated into modules based on the type of file that is to be analyzed. There are four modules: http://seqanswers.com/wiki/Software/list http://seqanswers.com/wiki/Bedutils Requirements Requires Mac OSX or Linux operating system (tested on RHEL5 and RHEL6) Python 2.6+ (including development packages) virtualenv Make Note: for RHEL5, it is recommended that you add the EPEL repository to your yum configuration and install the python26 and python26-virtualenv packages. This will let NGSUtils use the proper version of Python without disrupting any system utilities. http://ngsutils.org/installation/ http://ngsutils.org/ PS: LZ baozis ten please? if above package worked it for your ongoing project.