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Can someone recommend an oligo synthesis company?
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Can someone recommend an oligo synthesis company?# Biology - 生物学
a*u
1 楼
报喜一下。
刚刚收到USCIS的短信，485批了，card is ordered.
TSC EB-1B
Timeline:
I140 Approved on June 1th
I485 Receive Date: June 15th
I485 Notice Date: June 30th
FP: Aug 18th
EAD: Sep 10th
AP: Sep 15th
Card ordered: Sep 20th
485状态直接从acceptance跳到Card has been ordered.
等收到绿卡，花一个晚上好好写一下绿卡申请经历，供各位绿友参考。
avatar
D*9
2 楼
Microsoft Proposal to Add/Issue 20,000 More H-1B Visa Above Current Annual
Cap on $10,000 Filing Fees and 20,000 STEM Immigrant Visas on $15,000 Filing
Fees?
can pass?
avatar
p*1
3 楼
我们算不算免冠且美丽大方？现在的这个电脑照片就几张，我家猫们最富有免冠正面照
。。。
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N*n
4 楼
I have been having great difficulty doing subcloning. It used to be nothing
but now it is some kind of extreme work. I thought it was because my skill
deteriorated badly. Until I sequence a few clones. All the clones showed
that one of the two oligoes had lost half of the designed length. I thought
it was because the oligo wasn't pured and did a gel purification. To my
surprise, that particular oligo ran at the posistion corresponding to the
short size length compeletly. On the other hand, the other oligo ran
correctly. I have been buying my oligo from IDT all the time. Wasted so much
time. Now I have to find a different company for oligo synthesis.
Can someone recommend an oligo synthesis company that you have good
experience? Thanks very much fo the input.
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i*u
5 楼
gxgx
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y*q
6 楼
这将加大绿卡申请难度
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o*l
7 楼
好美丽!
这位是barney还是fred?

【在 p********1 的大作中提到】
: 我们算不算免冠且美丽大方？现在的这个电脑照片就几张，我家猫们最富有免冠正面照
: 。。。
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u*8
9 楼
congrats
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t*j
10 楼
Why

[发表自未名空间手机版 - m.mitbbs.com]

【在 y**q 的大作中提到】
: 这将加大绿卡申请难度
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A*R
11 楼
such a beauty!
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N*n
12 楼
idtdna is what I used and had problem with.

【在 p*****3 的大作中提到】
: IDT
: http://www.idtdna.com/site
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s*r
13 楼
GXGX. Good news!

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
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B*g
14 楼
俺们公司没钱给

【在 t***j 的大作中提到】
: Why
:
: [发表自未名空间手机版 - m.mitbbs.com]
avatar
s*r
15 楼
我知道我知道
哈哈
给我包子我告诉你

【在 o********l 的大作中提到】
: 好美丽!
: 这位是barney还是fred?
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a*d
16 楼
you may try genescript or genewiz
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c*e
17 楼
Congratulations!
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t*j
18 楼
不就１万块钱，你们不都年薪２０万吗？

[发表自未名空间手机版 - m.mitbbs.com]

【在 B*****g 的大作中提到】
: 俺们公司没钱给
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x*u
19 楼
面不够正
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f*h
21 楼
congratssss baoziiiii
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B*g
22 楼
那也不给，我们都是人才，必须免费

【在 t***j 的大作中提到】
: 不就１万块钱，你们不都年薪２０万吗？
:
: [发表自未名空间手机版 - m.mitbbs.com]
avatar
o*l
23 楼
-_-

我知道我知道
哈哈
给我包子我告诉你

【在 s******r 的大作中提到】
: 我知道我知道
: 哈哈
: 给我包子我告诉你
avatar
m*M
24 楼
多长的引物？怎么看出的少了一半？上面设有不配对的突变？如果老是这条有问题，而
另外一个是好的话，肯定是你自己的引物和模板那里不对。我用IDT从来没有什么问题
。
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f*h
25 楼
how about AP and EAD?
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S*r
26 楼
啥时候微软拥有美国国会议院席位了?
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Q*A
27 楼
好美啊！
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N*n
28 楼
It was only 32 nt. Based on sequencing result, it was short by 15nt. I tried
to purify the oligo on PAGE. It was short as well when compared to similar
length oligo. So I am sure it was short.
I have used idt for many years. If there were problem, I normally order news
one thinking my design had problem until I did sequencing and gel
purification.
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L*n
29 楼
congrats

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
p*f
30 楼
应该是barney.

【在 o********l 的大作中提到】
: 好美丽!
: 这位是barney还是fred?
avatar
m*M
31 楼
Can you describe it in detail? If 5' is missed, how did you clone the gene (
T-vector ligation)? If 15nt is missed at 3' how the primer complement with
your DNA plate correctly? If it complemented at right position how did you
know 15nt is missed sine it will be identical to one of your DNA strands?

tried
similar
news

【在 N****n 的大作中提到】
: It was only 32 nt. Based on sequencing result, it was short by 15nt. I tried
: to purify the oligo on PAGE. It was short as well when compared to similar
: length oligo. So I am sure it was short.
: I have used idt for many years. If there were problem, I normally order news
: one thinking my design had problem until I did sequencing and gel
: purification.
avatar
t*f
32 楼
congrats
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z*e
33 楼
太美了！
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N*n
34 楼
A PCR was performed and cloned directly into vecotr by ligation (primer
phosphorylated first). Sequencing indicated that one primer was missing 15nt
from the 5' end. Because the 3' end of the primer is intact, that is why
the PCR worked fine.

(

【在 m****M 的大作中提到】
: Can you describe it in detail? If 5' is missed, how did you clone the gene (
: T-vector ligation)? If 15nt is missed at 3' how the primer complement with
: your DNA plate correctly? If it complemented at right position how did you
: know 15nt is missed sine it will be identical to one of your DNA strands?
:
: tried
: similar
: news
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m*g
35 楼
GX
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c*n
36 楼

b和f最明显的区别就是右前抓的花纹不同
小马很狡猾，总不show出来

【在 o********l 的大作中提到】
: 好美丽!
: 这位是barney还是fred?
avatar
d*d
37 楼
恭喜恭喜,咱们俩timeline几乎差不多,希望我的这个星期也批!

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
o*5
38 楼
1/3侧面照
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P*x
39 楼
cong!

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
a*u
40 楼
bless!

【在 d*****d 的大作中提到】
: 恭喜恭喜,咱们俩timeline几乎差不多,希望我的这个星期也批!
avatar
d*d
41 楼
多谢你啊,咱们俩ap一天批的,你的ap收到了么?

【在 a******u 的大作中提到】
: bless!
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p*l
42 楼
CON
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a*u
43 楼
今天收到我领导的，我的还没到。

【在 d*****d 的大作中提到】
: 多谢你啊,咱们俩ap一天批的,你的ap收到了么?
avatar
i*u
44 楼
我比你晚一天批，今天收到

【在 d*****d 的大作中提到】
: 多谢你啊,咱们俩ap一天批的,你的ap收到了么?
avatar
d*d
45 楼
啊,就我还没收到了..我是上周二批的ap.
ead周六收到了.急等ap啊,sigh

【在 i******u 的大作中提到】
: 我比你晚一天批，今天收到
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k*e
46 楼
gx
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d*e
47 楼
GXGX!
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l*7
48 楼
GXGX
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A*d
49 楼
GXGX，中秋最好礼物呀
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A*T
50 楼
gxgx
移民局开始干活了

报喜一下。
刚刚收到USCIS的短信，485批了，card is ordered.
TSC EB-1B
Timeline:
I140 Approved on June 1th
I485 Receive Date: June 15th
I485 Notice Date: June 30th
FP: Aug 18th
EAD: Sep 10th
AP: Sep 15th
Card ordered: Sep 20th
485状态直接从acceptance跳到Card has been ordered.
等收到绿卡，花一个晚上好好写一下绿卡申请经历，供各位绿友参考。

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
w*c
51 楼
GXGX!
avatar
d*w
52 楼
cong

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
m*o
53 楼
big congrats,沾喜气

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
g*e
54 楼
gxgx

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
m*j
55 楼
few good news on 485 though, congratulations !

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
c*g
56 楼
恭喜恭喜！
看来TSC 485又开始干活了

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
K*o
57 楼
GXGX!
avatar
E*d
58 楼
恭喜！！

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
n*6
59 楼
GXGX... zhan zhan xi qi and bao zi please...

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
y*p
60 楼
恭喜，等经验
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a*a
61 楼
gongxigongxi
看来uscis真的开始work on EB1了
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H*e
62 楼
GX
avatar
l*y
63 楼
congrats!!!!!!!!!
avatar
G*0
64 楼
GXGX

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
s*r
65 楼
GX 还是EB-1B快
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y*a
66 楼
gxgx!
沾点喜气
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J*n
67 楼
congratulations.
avatar
l*j
68 楼
gxgx
avatar
l*t
69 楼
congrats
avatar
R*n
70 楼
GXGX
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T*2
71 楼
GX
avatar
T*2
72 楼
GX
avatar
a*5
73 楼
gx
avatar
d*2
74 楼
GXGX

报喜一下。
刚刚收到USCIS的短信，485批了，card is ordered.
TSC EB-1B
Timeline:
I140 Approved on June 1th
I485 Receive Date: June 15th
I485 Notice Date: June 30th
FP: Aug 18th
EAD: Sep 10th
AP: Sep 15th
Card ordered: Sep 20th
485状态直接从acceptance跳到Card has been ordered.
等收到绿卡，花一个晚上好好写一下绿卡申请经历，供各位绿友参考。

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
w*g
75 楼
恭喜恭喜！！

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
m*l
76 楼
恭喜恭喜.
沾点喜气.

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
h*l
77 楼
gx
avatar
b*9
78 楼
gx!
avatar
p*i
79 楼
恭喜恭喜
avatar
s*n
80 楼
GXGX!

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
d*o
81 楼
GX
avatar
p*o
82 楼
GXGX!
avatar
d*k
83 楼
cong
avatar
g*a
84 楼
GXGX!
avatar
s*y
85 楼
恭喜恭喜！
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z*o
86 楼
GXGX!!!
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o*b
87 楼
gxgx!
avatar
J*e
88 楼
gxgx
avatar
l*a
89 楼
re
avatar
y*o
90 楼
gx
avatar
a*l
91 楼
cong

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
s*g
92 楼
gxgx
avatar
q*g
93 楼
恭喜恭喜！！
看今天你们几个好像都是2个月左右，不奢望移民局能更快，只希望移民局能保持这个
效率就好了。
avatar
A*a
94 楼
gxgx
avatar
P*u
95 楼
GXGX!!!!!
avatar
d*c
96 楼
恭喜了！ 希望移民局加速干活，把我们的也快点批了！！

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
x*n
97 楼
cong~
avatar
d*o
98 楼
cong!
avatar
H*a
99 楼
恭喜！

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
avatar
c*b
100 楼
gxgx,又一个过关了。
avatar
i*m
101 楼
gx!
avatar
y*w
102 楼
cong!
avatar
t*y
103 楼
TSC就是快呀！gxgx,真希望能沾到喜气。
avatar
t*6
104 楼
gongxi!
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d*y
105 楼
gx!
avatar
d*s
106 楼
gxgx!
avatar
T*2
107 楼
gx
avatar
b*y
108 楼
cong

【在 a******u 的大作中提到】
: 报喜一下。
: 刚刚收到USCIS的短信，485批了，card is ordered.
: TSC EB-1B
: Timeline:
: I140 Approved on June 1th
: I485 Receive Date: June 15th
: I485 Notice Date: June 30th
: FP: Aug 18th
: EAD: Sep 10th
: AP: Sep 15th
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