s*s
2 楼
这跟用啥pull down没关系吧。你做cell extract当然都要加，
除非lysis buffer很强
【在 m******t 的大作中提到】
: in lysis buffer??
除非lysis buffer很强
【在 m******t 的大作中提到】
: in lysis buffer??
m*t
3 楼
sigma protocol does not mention protease inhibitor at all (i use sigma M2
beads).
and samples need to rotate at RT for 1 hour.
that is why i asked
beads).
and samples need to rotate at RT for 1 hour.
that is why i asked
T*u
4 楼
totally depends on the stablility of your protein.
【在 m******t 的大作中提到】
: sigma protocol does not mention protease inhibitor at all (i use sigma M2
: beads).
: and samples need to rotate at RT for 1 hour.
: that is why i asked
【在 m******t 的大作中提到】
: sigma protocol does not mention protease inhibitor at all (i use sigma M2
: beads).
: and samples need to rotate at RT for 1 hour.
: that is why i asked
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