求助个问题，如何改变glycolysis rate？ - 未名空间MITBBS历史存档

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求助个问题，如何改变glycolysis rate？

求助个问题，如何改变glycolysis rate？# Biology - 生物学

l*i2014-01-09 08:01

1 楼

我的情况是这样的。
和老公东西岸两地分居，两个宝宝跟着我。我跟着老公在2012年1月交了I-485（老公
PD 2008年9月），如今手头有comb EAD卡。我自己现在还是H1B身份，不过到6月H1B的
六年就到期了。现有一个在老公所在城市的大公司想要我，不知道要Transfer H1B，还
是直接用EAD得了。有以下疑问。
1） Option 1：用H1B.
由于我的6年H1B快到期了(我有自己的现在公司的perm和I-140)， H1B还能transfer到
新公司吗？以后还能延期吗？
2） Option 2：用EAD
如果用了EAD，我就失去了自己的H1B身份。又担心老公的NIW I-485被拒，那样我们两
个都没有身份了，就要回国了。
老公在拿到EAD后从academia转到了Industry，但还是在同一领域。从2012年1月份交
了485，拿到EAD，6月份跨州搬家，11月份renew EAD，都没有收到过RFE.大家觉得我
老公I485被拒的可能性有多大。
请版上的的xdjm们给些建议。谢谢！

K*R2014-01-09 08:01

2 楼

有什么方法能在实验中改变glycolysis rate？

f*u2014-01-09 08:01

3 楼

1，能，能
2，很小，

h*o2014-01-09 08:01

4 楼

in vitro or in vivo?
in vitro it is easy
You can use hexokinase inhibitor, such as 2-deoxyglucose,
You can use PDH activator, such as dicholoracetate
You can use Pyruvate transporter inhibitor, Thiazolidinediones
There are so many ways to manipulate cellular bioenergetic flux (glycolysis
or Mitochondrial OXPHOS), some may require digitonin or other similar
detergent to permeablize plasma membrane.

t*22014-01-09 08:01

5 楼

H1B, my company policy is using H1B

p*h2014-01-09 08:01

6 楼

2-DG is a substrate of HK, not an inhibitor. But it cannot be further
metabolized by downstream enzymes. In this sense, it inhibits glycolysis.
DCA is dirty. So is TZD.

glycolysis

【在 h*******o 的大作中提到】

: in vitro or in vivo?
: in vitro it is easy
: You can use hexokinase inhibitor, such as 2-deoxyglucose,
: You can use PDH activator, such as dicholoracetate
: You can use Pyruvate transporter inhibitor, Thiazolidinediones
: There are so many ways to manipulate cellular bioenergetic flux (glycolysis
: or Mitochondrial OXPHOS), some may require digitonin or other similar
: detergent to permeablize plasma membrane.

l*i2014-01-09 08:01

7 楼

新的公司和原来的perm和140都没有联系，真的可以帮我transfer或者extension吗？

h*o2014-01-09 08:01

8 楼

2-DG can be considered as a suicide inhibitor of hexokinase, its
phosphoproduct by hexokinase, 2DG6P, inhibits hexokinase activity.
When dealing with metabolic flux, there is nothing that comes clean, that's
why such experiments need to be tightly controlled with multiple inhibitors/
reagents targeting different steps of metabolic pathway.

【在 p*****h 的大作中提到】

: 2-DG is a substrate of HK, not an inhibitor. But it cannot be further
: metabolized by downstream enzymes. In this sense, it inhibits glycolysis.
: DCA is dirty. So is TZD.
:
: glycolysis

p*h2014-01-09 08:01

9 楼

Suicide inhibitor is a term reserved for those capable of covalent
modification of enzyme active site. Of course at high concentrations, 2DG6P
as a substrate /product analogue inhibits several glycolytic enzymes
including HK and PFK.
It is for historical reasons that these drugs are dirty (phenotypic drug
screening came well before rational design). This is not to say there will
be no better drugs.
在 hellozero (hellozero) 的大作中提到: 】
s
inhibitors/

l*12014-01-09 08:01

10 楼

p53+TIGAR
PMID 19015259
J Biol Chem. 2009 284: 1748-54.
Structural and biochemical studies of TIGAR (TP53-induced glycolysis and
apoptosis regulator).
http://www.ncbi.nlm.nih.gov/pubmed/19015259
or
PMID 23999443
J Clin Invest. 2013 123: 3685-92.
Targeting lactate metabolism for cancer therapeutics.
http://www.ncbi.nlm.nih.gov/pubmed/23999443

【在 K**R 的大作中提到】

: 有什么方法能在实验中改变glycolysis rate？

K*R2014-01-09 08:01

11 楼

暂时是invitro 多谢非常有用！

glycolysis

【在 h*******o 的大作中提到】

h*o2014-01-09 08:01

12 楼

any example of "clean" modulators of metabolic flux/glycolysis?

2DG6P

【在 p*****h 的大作中提到】

: Suicide inhibitor is a term reserved for those capable of covalent
: modification of enzyme active site. Of course at high concentrations, 2DG6P
: as a substrate /product analogue inhibits several glycolytic enzymes
: including HK and PFK.
: It is for historical reasons that these drugs are dirty (phenotypic drug
: screening came well before rational design). This is not to say there will
: be no better drugs.
: 在 hellozero (hellozero) 的大作中提到: 】
: s
: inhibitors/

h*o2014-01-09 08:01

13 楼

pigfish was right, 2-DG usually is not called as hexokinase inhibitor but
glycolytic inhibitor.
There are many other metabolic modulators that you can use, but keep in mind
, they often come with primary and secondary impact of metabolic flux and
you should tightly control your study so that the outcome can be interpreted
properly.

【在 K**R 的大作中提到】

: 暂时是invitro 多谢非常有用！
:
: glycolysis