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9kb gene rescue experiment
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9kb gene rescue experiment# Biology - 生物学
S*e
1 楼
a mutant has been mapped to a gene ~9kb. need to do rescue experiment to
confirm the gene specificity of phenotype. RNA injection apparently not
doable since its easy degradation. DNA injection mosaicism is not penetrated
enough to recover the whole phenotype. any suggestions?
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l*e
2 楼
crispr/cas9 mediated knockin repair

penetrated

【在 S*********e 的大作中提到】
: a mutant has been mapped to a gene ~9kb. need to do rescue experiment to
: confirm the gene specificity of phenotype. RNA injection apparently not
: doable since its easy degradation. DNA injection mosaicism is not penetrated
: enough to recover the whole phenotype. any suggestions?
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H*N
3 楼
in what species?
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