有没有啥荧光dye能只染活细菌，不染死细菌 - 未名空间MITBBS历史存档

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有没有啥荧光dye能只染活细菌，不染死细菌

有没有啥荧光dye能只染活细菌，不染死细菌# Biology - 生物学

k*g2014-02-04 08:02

1 楼

我的实验只要求染活细菌就够了，不需要把死细菌有多少测出来，有没有什么荧光dye
能只染活细菌，不染死细菌？还是说只有用live/dead viability kit才可以知道有没
有活细菌？如果只能用kit，哪位大牛能给推荐个便宜点的吗，怎么染细菌的我看都400
刀以上。。

k*g2014-02-04 08:02

2 楼

Calcein, AM
这个用在细菌上能只染活的么，效果如何

dye
400

【在 k***g 的大作中提到】

: 我的实验只要求染活细菌就够了，不需要把死细菌有多少测出来，有没有什么荧光dye
: 能只染活细菌，不染死细菌？还是说只有用live/dead viability kit才可以知道有没
: 有活细菌？如果只能用kit，哪位大牛能给推荐个便宜点的吗，怎么染细菌的我看都400
: 刀以上。。

k*g2014-02-04 08:02

3 楼

哪位大侠能给点建议么？谢谢了！

d*i2014-02-04 08:02

4 楼

The Bacteria Live/Dead Staining Kit enables a two-color fluorescent staining
on both live bacteria (green) and dead bacteria (red) using the two nucleic
acid dyes DMAO and EthD-III. DMAO is a green-fluorescent dye which stains
both live and dead bacteria with intact and damaged cell membranes while the
red-fluorescing EthD-III only stains dead bacteria with damaged cell
membranes. With an appropriate mixture of both dyes, viable bacteria with
intact cell membranes are stained green, whereas bacteria with damaged cell
membranes fluoresce red. The kit is applicable to most bacteria types for
viability and cytotoxicity studies, and suitable for fluorescence microscopy
and flow cytometry.
http://www.promokine.info/products/cell-analysis/livedead-cell-
http://edepot.wur.nl/21194

d*i2014-02-04 08:02

5 楼

google "calcein am staining bacteria"

k*g2014-02-04 08:02

6 楼

太感谢以上两位了！我目前用着PI呢，我再买个Calcein AM