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FACS compensation问题# Biology - 生物学
b*r
1
http://www.usatoday.com/story/news/politics/2013/04/25/schumer-
WASHINGTON -- Two senators working on a sweeping immigration bill said it's
not a question of whether it would pass the Senate, but by how much.
Sen. John McCain, R-Ariz., and Sen. Charles Schumer, D-N.Y., members of the
so-called Gang of Eight that crafted the immigration plan, said they
believed the bill could reach 70 votes in the Senate, sending a message to
the House of Representatives of the bill's overwhelming support.
"We're looking not to get 61 votes -- obviously that's the minimum. I'd like
to get ... a majority on both sides," Schumer said during a breakfast
Thursday hosted by The Christian Science Monitor.
"I think that's very doable," McCain added.
While Washington hasn't revamped the nation's immigration laws since
President Ronald Reagan signed a bill in 1986 that legalized up to 3 million
unauthorized immigrants, McCain and Schumer struck a confident tone
Thursday morning that they could get their bill through Congress.
The 844-page bill would allow the nation's 11 million unauthorized
immigrants to become U.S. citizens within 13 years, would add $8.5 billion
to further secure the nation's borders and would fundamentally alter the
nation's legal immigration system to bring in more skilled workers.
Several Republican members of Congress have tried to slow down the bill. And
Sen. Chuck Grassley, R-Iowa, the highest-ranking Republican on the Senate
Judiciary Committee, said the Boston Marathon bombings should prompt
lawmakers to more closely scrutinize the national security implications of
the Gang of Eight's immigration bill.
Schumer dismissed such talk, saying their bill "might've made a difference"
in the Boston case. Part of the bill requires the Department of Homeland
Security to implement a system to better track every time someone enters and
exits the United States, and that could have avoided one of the possible
missteps in the Boston case.
Tamerlan Tsarnaev, one of the suspects in the case who died during a chase
with police, was on a government watch list, but Schumer said U.S.
authorities were not fully aware of his trip to Russia's Caucasus in 2012
because his airline misspelled his name.
"Under our bill, the name would've been machine-read ... and they would've
known exactly who he was," Schumer said, referring to passport-reading
technology that could be implemented for all entries and exits under the
bill.
Both senators also fought back against suggestions that the pathway to
citizenship for unauthorized immigrants could be watered down. The bill
currently allows most of the nation's unauthorized immigrants to become U.S.
citizens in 13 years after they pass a criminal background check, pay taxes
, fees and $2,000 in fines, learn English and maintain employment.
McCain said proposals to grant a temporary legal status to the nation's
unauthorized immigrants, but not allow them to seek permanent residency or U
.S. citizenship, "offends our fundamental principles of fairness."
"Any attempt to say in the House that it will not have a path to citizenship
will be a non-starter," Schumer said. "I say that unequivocally, it will
not pass the Senate. I don't think it'll get a Democratic vote."
The Senate Judiciary Committee held three hearings on the bill over the past
week, and plan to start voting on the bill in early May. A bipartisan group
of House members is developing its own immigration that it hopes to unveil
in the coming weeks.
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f*c
2
侧院正在做水泥地,想顺便把那个木门换成铁门,去了HD没有,不知道哪里有卖。
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m*z
3
需要做EGFP,PE,APC,Pacific blue和PE-Cy7的共染FACS,不过不确定前三种荧光能
否compensate开?不知道EGFP的表达量会不会影响compensation?
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k*b
4
70不保险,75比较好
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g*e
5
网上看到HD有

【在 f*****c 的大作中提到】
: 侧院正在做水泥地,想顺便把那个木门换成铁门,去了HD没有,不知道哪里有卖。
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j*g
6
没什么问题
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c*e
7
多会参院投票?

70不保险,75比较好

【在 k*b 的大作中提到】
: 70不保险,75比较好
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c*p
8
看看local广告,很多小家族生意做这个的
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f*a
9
PE和EGFP的488 laser compensation很容易,APC是用633laser,和前面两个不存在
compensation 问题。
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b*d
10
6月.

【在 c*******e 的大作中提到】
: 多会参院投票?
:
: 70不保险,75比较好

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f*p
11
这哪是家呀,快变成jail了。。。
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w*n
12
single color 肯定要做的,

【在 m*****z 的大作中提到】
: 需要做EGFP,PE,APC,Pacific blue和PE-Cy7的共染FACS,不过不确定前三种荧光能
: 否compensate开?不知道EGFP的表达量会不会影响compensation?

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y*n
13
70是保守估计,起码80票!
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f*c
14
我在店里,回去看看

【在 g***e 的大作中提到】
: 网上看到HD有
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m*z
15
谢谢。是前两个。我的不同细胞株的gfp表达量有高有低,这样compensate起来没问题
吧?用表达高的株做的也能适用于表达低的吧?

【在 f*******a 的大作中提到】
: PE和EGFP的488 laser compensation很容易,APC是用633laser,和前面两个不存在
: compensation 问题。

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f*c
16
好,谢谢

【在 c***p 的大作中提到】
: 看看local广告,很多小家族生意做这个的
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f*a
17
你的仪器有561 laser吗?如果是这样就可以不用compensation PE (561)和GFP(488),
最方便。
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f*c
18
这只臭苍蝇,几天没被拍,皮痒了?

【在 f****p 的大作中提到】
: 这哪是家呀,快变成jail了。。。
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