没什么可怕的,对于需要器官移植的人来说,是梦寐以求的。
Nature Medecine. 2008 Feb;14(2):213-21. Epub 2008 Jan 13.
Perfusion-decellularized matrix: using nature's platform to engineer a
bioartificial heart.
Ott HC, Matthiesen TS, Goh SK, Black LD, Kren SM, Netoff TI, Taylor DA.
Department of Surgery, Massachusetts General Hospital, Harvard Medical
School, 55 Fruit Street, Boston, Massachusetts 02114, USA.
Abstract
About 3,000 individuals in the United States are awaiting a donor heart;
worldwide, 22 million individuals are living with heart failure. A
bioartificial heart is a theoretical alternative to transplantation or
mechanical left ventricular support. Generating a bioartificial heart
requires engineering of cardiac architecture, appropriate cellular
constituents and pump function. We decellularized hearts by coronary
perfusion with detergents, preserved the underlying extracellular matrix,
and produced an acellular, perfusable vascular architecture, competent
acellular valves and intact chamber geometry. To mimic cardiac cell
composition, we reseeded these constructs with cardiac or endothelial cells.
To establish function, we maintained eight constructs for up to 28 d by
coronary perfusion in a bioreactor that simulated cardiac physiology. By day
4, we observed macroscopic contractions. By day 8, under physiological load
and electrical stimulation, constructs could generate pump function (
equivalent to about 2% of adult or 25% of 16-week fetal heart function) in a
modified working heart preparation.