w*g
2 楼
Samsung Note 2 for $149.99 after Walmart Rebate
Walmart wireless has the Note 2 for $249.99 on ATT with a $100 Walmart Gift
Card for a total of $149.99. Free shipping and no activation.
http://slickdeals.net/f/5558693-Samsung-Note-2-for-149-99-after
Walmart wireless has the Note 2 for $249.99 on ATT with a $100 Walmart Gift
Card for a total of $149.99. Free shipping and no activation.
http://slickdeals.net/f/5558693-Samsung-Note-2-for-149-99-after
f*r
3 楼
最近可能需要做个简单的proliferation assay,就是转染个基因然后几天过后数细胞数
量。据说转染率在80%左右。首先其实想问的就是做这个试验需要注意什么么？
其次，如果结果阴性的话，还有必要作cell death assay么？我记得曾经读过一篇文章
，说一个东西对proliferation没影响，但对cell death有影响（忘了他们是用flow还
是什么了）。板上有前辈能解释一下cell death assay一般怎么做的么？谢谢。
量。据说转染率在80%左右。首先其实想问的就是做这个试验需要注意什么么？
其次，如果结果阴性的话，还有必要作cell death assay么？我记得曾经读过一篇文章
，说一个东西对proliferation没影响，但对cell death有影响（忘了他们是用flow还
是什么了）。板上有前辈能解释一下cell death assay一般怎么做的么？谢谢。
y*0
4 楼
正常.
这个问题今后几个月会成为日经贴了...
这个问题今后几个月会成为日经贴了...
T*e
5 楼
thanks for the info, I am using t mobile monthly 4g $30 plan,but the signal
is not good at my work, so want to switch.
Anyway, I ordered the phone with only 3G data per month (up to 5G total, but
only the initial 3G is high speed) for $35, there is no minutes at all.
I think there will be a number, anyone knows how to make internet calls,
such as through google voice or skype? I have few calls, but is hoping that
there is a way for this to work. If not, I will have to cancel the plan in 2
weeks.
Thanks a lot!
Gift
【在 w******g 的大作中提到】
: Samsung Note 2 for $149.99 after Walmart Rebate
: Walmart wireless has the Note 2 for $249.99 on ATT with a $100 Walmart Gift
: Card for a total of $149.99. Free shipping and no activation.
: http://slickdeals.net/f/5558693-Samsung-Note-2-for-149-99-after
is not good at my work, so want to switch.
Anyway, I ordered the phone with only 3G data per month (up to 5G total, but
only the initial 3G is high speed) for $35, there is no minutes at all.
I think there will be a number, anyone knows how to make internet calls,
such as through google voice or skype? I have few calls, but is hoping that
there is a way for this to work. If not, I will have to cancel the plan in 2
weeks.
Thanks a lot!
Gift
【在 w******g 的大作中提到】
: Samsung Note 2 for $149.99 after Walmart Rebate
: Walmart wireless has the Note 2 for $249.99 on ATT with a $100 Walmart Gift
: Card for a total of $149.99. Free shipping and no activation.
: http://slickdeals.net/f/5558693-Samsung-Note-2-for-149-99-after
a*r
6 楼
cell death detection kit, sigma有卖的
好像是DNA 降解
你去sigma网上看吧，很简单的实验
好像是DNA 降解
你去sigma网上看吧，很简单的实验
t*r
7 楼
1 Y
f*r
8 楼
那如果proliferation assay（数细胞）结果阴性，还有必要作cell death assay么？
【在 a******r 的大作中提到】
: cell death detection kit, sigma有卖的
: 好像是DNA 降解
: 你去sigma网上看吧，很简单的实验
【在 a******r 的大作中提到】
: cell death detection kit, sigma有卖的
: 好像是DNA 降解
: 你去sigma网上看吧，很简单的实验
x*d
9 楼
做一下，排除transgene导致的death rate比proliferation rate快。
【在 f**r 的大作中提到】
: 那如果proliferation assay（数细胞）结果阴性，还有必要作cell death assay么？
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