正常。SDS is pretty sensitive to salt and Temperature. the optimal temperature we have optimized for 1%sds sonication is 17 degree. Others have reported this too. Even if people claimed 4degree sonication (pump-cold water circulation), the actual temperature is ~17degree. We always dissolve nuclei in lysis buffer right before the sonication to avoid the long stand of 1%sds+nuclei on ice, which will precipitate.
devil is in the details. 果然是高人! 那循环水的温度,我是应该设成4C还是17C呢? 我每个cycle 用15sec ON / 45sec OFF,到cycle后期似乎就能看到一点点SDS沉淀开始 析出。 多谢指教!
degree.
【在 g*********3 的大作中提到】 : 正常。SDS is pretty sensitive to salt and Temperature. : the optimal temperature we have optimized for 1%sds sonication is 17 degree. : Others have reported this too. : Even if people claimed 4degree sonication (pump-cold water circulation), : the actual temperature is ~17degree. : We always dissolve nuclei in lysis buffer right before the sonication to : avoid the long stand of 1%sds+nuclei on ice, which will precipitate. : : 10