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请教1 Ligation reaction question
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请教1 Ligation reaction question# Biology - 生物学
w*o
1
We can add a 5'-PO4-DNA (Single Stranded) to the 3'-end of a ssRNA using T4
RNA ligase I, but cannot add a 5'-PO4-RNA (single Stranded) to 3'-end of
ssDNA.
Highly appreciated any suggestion on how to add a ssRNA to a ssDNA. Thanks a
lot
avatar
t*a
2
Try this?
https://www.neb.com/protocols/2014/10/27/ligation-of-a-dna-or-rna-oligo-to-
single-stranded-rna-using-t4-rna-ligase-1

T4
a

【在 w***o 的大作中提到】
: We can add a 5'-PO4-DNA (Single Stranded) to the 3'-end of a ssRNA using T4
: RNA ligase I, but cannot add a 5'-PO4-RNA (single Stranded) to 3'-end of
: ssDNA.
: Highly appreciated any suggestion on how to add a ssRNA to a ssDNA. Thanks a
: lot

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