c*y
2 楼
r.t.谢谢
p*x
3 楼
我的信用卡额度是750，但是现在需要付一个800的东西，我可否预存50然后划卡，如果
超额划卡会怎样？
超额划卡会怎样？
A*o
4 楼
大概10万的纯收入，是否可以用turbo 的home and business软件直接自己报税？如果
欠税的话（家庭报税），是用个人支票交还是用公司的支票交呢？
欠税的话（家庭报税），是用个人支票交还是用公司的支票交呢？
s*e
5 楼
请问：J1 transfer 的过程中, 国内的J2能用以前的DS2019表去签证吗？
我目前的地方明天工作结束了，DS2019表的release date 是July 21. 新的地方工作开
始是July 23，但是我月底才回去新的地方。
我老公现在持有我目前学校的DS2019表，上面的end date 是2013年。我们现在还没有
新的2019表，不知道我老公持这个2019表去签证，有没有什么问题？
谢谢
我目前的地方明天工作结束了，DS2019表的release date 是July 21. 新的地方工作开
始是July 23，但是我月底才回去新的地方。
我老公现在持有我目前学校的DS2019表，上面的end date 是2013年。我们现在还没有
新的2019表，不知道我老公持这个2019表去签证，有没有什么问题？
谢谢
w*e
6 楼
最近喜欢听民乐，尝试写了一首歌：
https://soundcloud.com/yao-meng-1/c5jvkiq634qg
长安别
晨钟破初晓 灞柳穿古道
空看雁飞去 归期仍渺渺
暮鼓满城月 飘落渐成雪
楼台未登高 却见人已老
灯火阑珊处 琴瑟又笛箫
声声绕角羽 怕是离别调
无关风与月 起身和一曲
故人湿罗巾 只道琴声好
高山深谷 水涧轻舟
此间少年 一去白头
少时不归 归去不为
长安一别 千古悠悠
https://soundcloud.com/yao-meng-1/c5jvkiq634qg
长安别
晨钟破初晓 灞柳穿古道
空看雁飞去 归期仍渺渺
暮鼓满城月 飘落渐成雪
楼台未登高 却见人已老
灯火阑珊处 琴瑟又笛箫
声声绕角羽 怕是离别调
无关风与月 起身和一曲
故人湿罗巾 只道琴声好
高山深谷 水涧轻舟
此间少年 一去白头
少时不归 归去不为
长安一别 千古悠悠
N*D
7 楼
【 以下文字转载自 WaterWorld 讨论区 】
发信人: NYPD (New York, Pursuit of Dream), 信区: WaterWorld
标 题: Antibody Kills 91% of HIV Strains
发信站: BBS 未名空间站 (Thu Jul 8 16:02:53 2010, 美东)
from wsj
In a significant step toward an AIDS vaccine, U.S. government scientists
have discovered three powerful antibodies, the strongest of which
neutralizes 91% of HIV strains, more than any AIDS antibody yet discovered.
Looking closely at the strongest antibody, they have detailed exactly what
part of the virus it targets and
发信人: NYPD (New York, Pursuit of Dream), 信区: WaterWorld
标 题: Antibody Kills 91% of HIV Strains
发信站: BBS 未名空间站 (Thu Jul 8 16:02:53 2010, 美东)
from wsj
In a significant step toward an AIDS vaccine, U.S. government scientists
have discovered three powerful antibodies, the strongest of which
neutralizes 91% of HIV strains, more than any AIDS antibody yet discovered.
Looking closely at the strongest antibody, they have detailed exactly what
part of the virus it targets and
d*O
8 楼
year 2002
Volume 84
Issue 7
pages 1353–1362
http://onlinelibrary.wiley.com/doi/10.1002/app.10243/pdf
Thanks again!
Volume 84
Issue 7
pages 1353–1362
http://onlinelibrary.wiley.com/doi/10.1002/app.10243/pdf
Thanks again!
x*n
9 楼
ashr？
【在 w*****y 的大作中提到】
: 谢谢
【在 w*****y 的大作中提到】
: 谢谢
c*u
10 楼
blender
我就用的walmart的几块钱的那种手提式的
我就用的walmart的几块钱的那种手提式的
i*4
11 楼
不能预存 大部分情况
如果是店里你可以付100 刷700
如果直接刷800 有可能会被信用卡公司锯掉（为保护你的卡不被盗用)
如果是店里你可以付100 刷700
如果直接刷800 有可能会被信用卡公司锯掉（为保护你的卡不被盗用)
c*e
12 楼
这里貌似没人了.....
N*D
13 楼
重大突破啊同学们！
【在 N**D 的大作中提到】
: 【 以下文字转载自 WaterWorld 讨论区 】
: 发信人: NYPD (New York, Pursuit of Dream), 信区: WaterWorld
: 标 题: Antibody Kills 91% of HIV Strains
: 发信站: BBS 未名空间站 (Thu Jul 8 16:02:53 2010, 美东)
: from wsj
: In a significant step toward an AIDS vaccine, U.S. government scientists
: have discovered three powerful antibodies, the strongest of which
: neutralizes 91% of HIV strains, more than any AIDS antibody yet discovered.
: Looking closely at the strongest antibody, they have detailed exactly what
: part of the virus it targets and
【在 N**D 的大作中提到】
: 【 以下文字转载自 WaterWorld 讨论区 】
: 发信人: NYPD (New York, Pursuit of Dream), 信区: WaterWorld
: 标 题: Antibody Kills 91% of HIV Strains
: 发信站: BBS 未名空间站 (Thu Jul 8 16:02:53 2010, 美东)
: from wsj
: In a significant step toward an AIDS vaccine, U.S. government scientists
: have discovered three powerful antibodies, the strongest of which
: neutralizes 91% of HIV strains, more than any AIDS antibody yet discovered.
: Looking closely at the strongest antibody, they have detailed exactly what
: part of the virus it targets and
w*y
15 楼
A股的代码
【在 x*********n 的大作中提到】
: ashr？
【在 x*********n 的大作中提到】
: ashr？
u*r
16 楼
mixer
便宜的也OK，功率不要太小就好了
【在 c***y 的大作中提到】
: r.t.谢谢
便宜的也OK，功率不要太小就好了
【在 c***y 的大作中提到】
: r.t.谢谢
H*H
17 楼
好好听。 韵美词也美
【在 w*********e 的大作中提到】
: 最近喜欢听民乐，尝试写了一首歌：
: https://soundcloud.com/yao-meng-1/c5jvkiq634qg
: 长安别
: 晨钟破初晓 灞柳穿古道
: 空看雁飞去 归期仍渺渺
: 暮鼓满城月 飘落渐成雪
: 楼台未登高 却见人已老
: 灯火阑珊处 琴瑟又笛箫
: 声声绕角羽 怕是离别调
: 无关风与月 起身和一曲
【在 w*********e 的大作中提到】
: 最近喜欢听民乐，尝试写了一首歌：
: https://soundcloud.com/yao-meng-1/c5jvkiq634qg
: 长安别
: 晨钟破初晓 灞柳穿古道
: 空看雁飞去 归期仍渺渺
: 暮鼓满城月 飘落渐成雪
: 楼台未登高 却见人已老
: 灯火阑珊处 琴瑟又笛箫
: 声声绕角羽 怕是离别调
: 无关风与月 起身和一曲
a*a
18 楼
最后一句很汗
【在 N**D 的大作中提到】
: 重大突破啊同学们！
【在 N**D 的大作中提到】
: 重大突破啊同学们！
x*n
19 楼
A股水深，不敢乱买
【在 w*****y 的大作中提到】
: A股的代码
【在 w*****y 的大作中提到】
: A股的代码
c*y
20 楼
magic bullet blender可以用来打发蛋白吗？
【在 u******r 的大作中提到】
: mixer
: 便宜的也OK，功率不要太小就好了
【在 u******r 的大作中提到】
: mixer
: 便宜的也OK，功率不要太小就好了
y*1
21 楼
Bullshit
u*r
22 楼
呃 这个东西我不知道。。。
但是一般用这个呀
http://tinyurl.com/ycdyh7b
但是一般用这个呀
http://tinyurl.com/ycdyh7b
s*y
23 楼
I just read this on my cellphone.
It is likely an antibody against a conserved region of the virus.
Such a concept exists for a long time but I am suprised it took so
long to produce something like that for HIV
【在 N**D 的大作中提到】
: 重大突破啊同学们！
It is likely an antibody against a conserved region of the virus.
Such a concept exists for a long time but I am suprised it took so
long to produce something like that for HIV
【在 N**D 的大作中提到】
: 重大突破啊同学们！
r*3
24 楼
I use KitchenAid stand mixer. It' s perfect!
a*a
25 楼
之前还有个号称治aids的发了nature biotechnology，不知道是不是就是这个
【在 s******y 的大作中提到】
: I just read this on my cellphone.
: It is likely an antibody against a conserved region of the virus.
: Such a concept exists for a long time but I am suprised it took so
: long to produce something like that for HIV
【在 s******y 的大作中提到】
: I just read this on my cellphone.
: It is likely an antibody against a conserved region of the virus.
: Such a concept exists for a long time but I am suprised it took so
: long to produce something like that for HIV
l*j
26 楼
walmart最便宜的mixer就足够了，blender不可以
s*y
27 楼
Actually it is not that difficult as we may think
For example we can figure out the binding pocket of the antibody and then
either make it a monoclonal mini-antibody for injection or make the DNA
for generation of such a antibody and clone it into our bone marrow stem
cells
【在 a***a 的大作中提到】
: 最后一句很汗
For example we can figure out the binding pocket of the antibody and then
either make it a monoclonal mini-antibody for injection or make the DNA
for generation of such a antibody and clone it into our bone marrow stem
cells
【在 a***a 的大作中提到】
: 最后一句很汗
a*a
28 楼
单抗可行，第二个基本现阶段是扯，是科幻小说
【在 s******y 的大作中提到】
: Actually it is not that difficult as we may think
: For example we can figure out the binding pocket of the antibody and then
: either make it a monoclonal mini-antibody for injection or make the DNA
: for generation of such a antibody and clone it into our bone marrow stem
: cells
【在 s******y 的大作中提到】
: Actually it is not that difficult as we may think
: For example we can figure out the binding pocket of the antibody and then
: either make it a monoclonal mini-antibody for injection or make the DNA
: for generation of such a antibody and clone it into our bone marrow stem
: cells
h*n
29 楼
最后一段都很汗.
【在 a***a 的大作中提到】
: 最后一句很汗
【在 a***a 的大作中提到】
: 最后一句很汗
s*y
30 楼
How come? Such thing can be done in vitro in a rather straight forward way.
Can we transfect the donor bone marrow cells and then go for immuno
stimulate
and selection and finally select for the memory B cell that express that
particular antibody and put it back to the donor's body?
I mean there will be a lot of work invovled but in theory that should be
doable
right?
【在 a***a 的大作中提到】
: 单抗可行，第二个基本现阶段是扯，是科幻小说
Can we transfect the donor bone marrow cells and then go for immuno
stimulate
and selection and finally select for the memory B cell that express that
particular antibody and put it back to the donor's body?
I mean there will be a lot of work invovled but in theory that should be
doable
right?
【在 a***a 的大作中提到】
: 单抗可行，第二个基本现阶段是扯，是科幻小说
a*a
31 楼
thousands of things can be done in vitro.BUT....
【在 s******y 的大作中提到】
: How come? Such thing can be done in vitro in a rather straight forward way.
: Can we transfect the donor bone marrow cells and then go for immuno
: stimulate
: and selection and finally select for the memory B cell that express that
: particular antibody and put it back to the donor's body?
: I mean there will be a lot of work invovled but in theory that should be
: doable
: right?
【在 s******y 的大作中提到】
: How come? Such thing can be done in vitro in a rather straight forward way.
: Can we transfect the donor bone marrow cells and then go for immuno
: stimulate
: and selection and finally select for the memory B cell that express that
: particular antibody and put it back to the donor's body?
: I mean there will be a lot of work invovled but in theory that should be
: doable
: right?
p*m
32 楼
其实都用不了这么麻烦 直接在造血干细胞里面突变掉CCR5然后原封不动的给患者注射回
去就行了 也不需要考虑哪个antibody好用 呵呵
其实拿bone marrow cell出来转了基因然后种回去已经做了n个临床实验了 效果不好说
但是至少是feasible的
【在 s******y 的大作中提到】
: How come? Such thing can be done in vitro in a rather straight forward way.
: Can we transfect the donor bone marrow cells and then go for immuno
: stimulate
: and selection and finally select for the memory B cell that express that
: particular antibody and put it back to the donor's body?
: I mean there will be a lot of work invovled but in theory that should be
: doable
: right?
去就行了 也不需要考虑哪个antibody好用 呵呵
其实拿bone marrow cell出来转了基因然后种回去已经做了n个临床实验了 效果不好说
但是至少是feasible的
【在 s******y 的大作中提到】
: How come? Such thing can be done in vitro in a rather straight forward way.
: Can we transfect the donor bone marrow cells and then go for immuno
: stimulate
: and selection and finally select for the memory B cell that express that
: particular antibody and put it back to the donor's body?
: I mean there will be a lot of work invovled but in theory that should be
: doable
: right?
K*S
33 楼
"Researchers screened 25 million of his cells
to find 12 that produced the antibodies. Now the trick will be for
scientists to develop a vaccine or other methods to make anyone's body
produce them."
now they will keep that man as national treasure.
【在 N**D 的大作中提到】
: 重大突破啊同学们！
to find 12 that produced the antibodies. Now the trick will be for
scientists to develop a vaccine or other methods to make anyone's body
produce them."
now they will keep that man as national treasure.
【在 N**D 的大作中提到】
: 重大突破啊同学们！
W*C
34 楼
the strongest of which neutralizes 91% of HIV strains，more than any AIDS
antibody yet discovered.
ｃｏｎｔｒｏｌ做得不好， 之前的抗体是９０％呢还是１％呢？
antibody yet discovered.
ｃｏｎｔｒｏｌ做得不好， 之前的抗体是９０％呢还是１％呢？
s*3
35 楼
I just read this article. It seems pretty good.
In the last figure, they compare the neutralization potency and breadth of
the new antibody VRC01 with that of b12, a well-known broadly neutralizing
Ab against CD4 binding site of gp120. Under IC50 <50 ug/ml, VRC01 can
neutralize 91% of 190 Env pseudoviruses, whereas b12 can only neutralize 41%.
One question about the paper is that they design a molecule, RSC3, that
keeps the antigenicity of CD4 binding site of gp120 whereas remove the
antigenicit
In the last figure, they compare the neutralization potency and breadth of
the new antibody VRC01 with that of b12, a well-known broadly neutralizing
Ab against CD4 binding site of gp120. Under IC50 <50 ug/ml, VRC01 can
neutralize 91% of 190 Env pseudoviruses, whereas b12 can only neutralize 41%.
One question about the paper is that they design a molecule, RSC3, that
keeps the antigenicity of CD4 binding site of gp120 whereas remove the
antigenicit
O*e
36 楼
Or you should say: thousands of things were claimed to happen in vitro....
【在 a***a 的大作中提到】
: thousands of things can be done in vitro.BUT....
【在 a***a 的大作中提到】
: thousands of things can be done in vitro.BUT....
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