Re: dummy questions__how to get small DNA pieces?# Biology - 生物学
m*g
1 楼
1. buy enough amount of commercially available herring (or salmon) sperm DNA.
They are dirty cheap. you can get 100mg/$200 probably anywhere.
2. digest your DNA using AluI restriction enzyme. If AluI does not work
well, try other enzymes that recognize 4-nucleotide palingdromic sequence.
You may combine 2 enzymes in one digestion mixture.
3. run a 6% denatured sequencing gel using a thicker spacer set (like 1mm), or
better (maybe) a 1.5% agarose gel to seperate your DNA.
4. Cut out y
They are dirty cheap. you can get 100mg/$200 probably anywhere.
2. digest your DNA using AluI restriction enzyme. If AluI does not work
well, try other enzymes that recognize 4-nucleotide palingdromic sequence.
You may combine 2 enzymes in one digestion mixture.
3. run a 6% denatured sequencing gel using a thicker spacer set (like 1mm), or
better (maybe) a 1.5% agarose gel to seperate your DNA.
4. Cut out y