Re: 有人在细菌里表达过cysteine rich protein吗？# Biology - 生物学
r*o
1 楼
Do you have a way to distinguish correctly folded protein? If you have,
just use HPLC to purify them from crude E.Coli expressed protein.
If you don't have one way to do that.
Refolding involves (1) denaturing and reducing (2) suddently dilute
the sample extensively to mild condition (3) get the soluble protein.
Try to search pubmed for methods or refer to any classic protein biochemistry
protocol book. Note: the yield is low(less than 10% of the crude protein
will be correctly refolded).
just use HPLC to purify them from crude E.Coli expressed protein.
If you don't have one way to do that.
Refolding involves (1) denaturing and reducing (2) suddently dilute
the sample extensively to mild condition (3) get the soluble protein.
Try to search pubmed for methods or refer to any classic protein biochemistry
protocol book. Note: the yield is low(less than 10% of the crude protein
will be correctly refolded).