Re: anyone working on RNA 2nd structure folding?# Biology - 生物学
t*u
1 楼
It really depends on the GC content of your RNA. My suggestion is to shuffle
and generate a random pool (say, 100) of your RNA based on the GC content.
Then run them through mfold (the server can handle batch jobs, you just need
to wait for a while), and then you will have a normal distribution of the
folding free energy. Compare the folding free energy of your native sequence
to the distribution will show you whether it's better or worse than random.
and generate a random pool (say, 100) of your RNA based on the GC content.
Then run them through mfold (the server can handle batch jobs, you just need
to wait for a while), and then you will have a normal distribution of the
folding free energy. Compare the folding free energy of your native sequence
to the distribution will show you whether it's better or worse than random.