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Re: SOS! 请教如何防止蛋白质在sample buffer 中沉淀
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Re: SOS! 请教如何防止蛋白质在sample buffer 中沉淀# Biology - 生物学
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Good point!
I am sure that the precipition was caused by potassium ions which complex with
SDS and become insoluble. You should remove potassium ions from your SDS
extraction solution by using sodium salt.
I do think your protein concentration is too high because I even used much
higher protein concentration for my protein 2d electrophoresis. But for
SDS-PAGE, you can not load too much protein even with a concentrated sample.
You still have to dilute your sample. I usually load 20-30 ug for my
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