Re: How long does it take for expression - 未名空间MITBBS历史存档

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Re: How long does it take for expression

Re: How long does it take for expression# Biology - 生物学

m*o2004-03-02 08:03

1 楼

The time for assay your transient expression depends on your host cell, transcrioption
effeciency,posttranslation mechanism etc. In one words, there are many may have
effect on your expression assay. So people used to do a progressive assay to
determine the best time point for your assay.
According to different transfection protocol, there is difference of incubation
time before conducting the assay.
The mechanism of transfection between Lipo and Calcium is diferent. For the
different cases, the

e*a2004-03-02 08:03

2 楼

Maybe you can try different protocals by using only EGFP, and check the cells
at differnt time points ,you will be able to see the EGFP expression by
noticing the green cells under fluorenscent microscope, so that you are able
to tell the time course of plasmid expression in different protocals.
And different plasmids have differnt expression patterns, I would try to
harvest transfected cells at different time points and measure the protein
level.
usually people harvest transient transfected cel