Re: 蛋白纯化的脱盐问题# Biology - 生物学
f*p
1 楼
desalting column is much faster, but sometimes proteins bind to it
non-specifically and you may lose some of your sample by that. sometimes,
proteins get denatured because the buffer change is too sudden, not like
dialysis that is slow and "tender". plus, carry over is almost inevitable on
the column, and a 2nd run is sometimes necessary.
dialysis on the other hand, has its disadvantage too. most of all, it takes
way too long (O/N is routine), during which the protein may not be happy.
second, i
non-specifically and you may lose some of your sample by that. sometimes,
proteins get denatured because the buffer change is too sudden, not like
dialysis that is slow and "tender". plus, carry over is almost inevitable on
the column, and a 2nd run is sometimes necessary.
dialysis on the other hand, has its disadvantage too. most of all, it takes
way too long (O/N is routine), during which the protein may not be happy.
second, i