h*u
2 楼
Try to treat cells with a chemical. This chemical is easy to dissolve in
DMSO. However, if adding to culture meidum, it precipiates.
How to solve this problem? Lower the concentration?
Thanks~~~~~~~~
DMSO. However, if adding to culture meidum, it precipiates.
How to solve this problem? Lower the concentration?
Thanks~~~~~~~~
v*r
3 楼
ding.
【在 v*****r 的大作中提到】
: rt, thanks
【在 v*****r 的大作中提到】
: rt, thanks
s*y
4 楼
Don't add the chemical to the medium,
instead, add the medium to the chemical.
For example, add the chemical to a empty 50mL tube, then quickly add 10mL
media to disperse the chemical. This should help
【在 h******u 的大作中提到】
: Try to treat cells with a chemical. This chemical is easy to dissolve in
: DMSO. However, if adding to culture meidum, it precipiates.
: How to solve this problem? Lower the concentration?
: Thanks~~~~~~~~
instead, add the medium to the chemical.
For example, add the chemical to a empty 50mL tube, then quickly add 10mL
media to disperse the chemical. This should help
【在 h******u 的大作中提到】
: Try to treat cells with a chemical. This chemical is easy to dissolve in
: DMSO. However, if adding to culture meidum, it precipiates.
: How to solve this problem? Lower the concentration?
: Thanks~~~~~~~~
Y*3
5 楼
10/18
r*e
6 楼
请问是什么原理？
局部浓度太大？
【在 s******y 的大作中提到】
: Don't add the chemical to the medium,
: instead, add the medium to the chemical.
: For example, add the chemical to a empty 50mL tube, then quickly add 10mL
: media to disperse the chemical. This should help
局部浓度太大？
【在 s******y 的大作中提到】
: Don't add the chemical to the medium,
: instead, add the medium to the chemical.
: For example, add the chemical to a empty 50mL tube, then quickly add 10mL
: media to disperse the chemical. This should help
y*u
7 楼
我看iflychina上面的信息说，有人9月20号的时候预约到了10月25号的。
我昨天替父母预约时，约的是10月29号。不过那是我们自己指定的时间，没问最早可以
约到哪一天，我猜再往前几天应该也可以。
我昨天替父母预约时，约的是10月29号。不过那是我们自己指定的时间，没问最早可以
约到哪一天，我猜再往前几天应该也可以。
s*y
8 楼
Yes.
【在 r***e 的大作中提到】
: 请问是什么原理？
: 局部浓度太大？
【在 r***e 的大作中提到】
: 请问是什么原理？
: 局部浓度太大？
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