如何把lymphblasts固定在slides上 - 未名空间MITBBS历史存档

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如何把lymphblasts固定在slides上

如何把lymphblasts固定在slides上# Biology - 生物学

l*a2011-01-11 08:01

1 楼

We have patient lymphblast cell line and want to see if some mRNA are stuck
in the nucleus. our plan is to In situ hybridyzation using this cell line.
But the problem is how to fix the cells on slides?
Any suggestion? Thanks a lot

s*s2011-01-11 08:01

2 楼

贴壁的细胞，直接把slide扔到dish里面长；悬浮的细胞，
用cytospin。

stuck

【在 l*****a 的大作中提到】

: We have patient lymphblast cell line and want to see if some mRNA are stuck
: in the nucleus. our plan is to In situ hybridyzation using this cell line.
: But the problem is how to fix the cells on slides?
: Any suggestion? Thanks a lot

l*a2011-01-11 08:01

3 楼

Thanks Shakuras! Can you tell a bit more about cytospin?

s*s2011-01-11 08:01

4 楼

我没用过。应该就是一些装置方便你把细胞离心到slides上 google吧

【在 l*****a 的大作中提到】

: Thanks Shakuras! Can you tell a bit more about cytospin?

s*g2011-01-11 08:01

5 楼

我这里有这种离心机.Shandon的cytospin3.离心时把悬浮细胞液(~100uL)放到Single
cyto Funnel w/card (Biomedical ploymers, INC)(REF:BMP-CYTO-S50)(聚集细胞和吸
干作用液体),而slide就在Funnel下收集细胞
就是把悬浮细胞离心后,贴在slide上成个绿豆大的面积.然后固定/染色....

l*a2011-01-11 08:01

6 楼

Thank you shyoung. found one cytospin in our institute.