n*d
2 楼
随便google了一下, 有好多, 不知道那家比较好.
有用过提供ISH服务的公司嘛? 多谢
有用过提供ISH服务的公司嘛? 多谢
w*u
5 楼
cell? section? embryo?
有时间还是自己做吧,公司其实也都不可靠。
出钱我帮你做也行,^_^!
有时间还是自己做吧,公司其实也都不可靠。
出钱我帮你做也行,^_^!
m*n
6 楼
为毛要找公司做。。。==|||||| 有你找公司的时间自己都能实验好几个probes了。。
s*9
7 楼
I am also doing in situ with DIG probe. None of the probes from my designs
show any signal while other probes from other lab works very well as
positive control. Do you have any suggestion for probe design?
The mRNA is 1.5kb. I desgined three probes, full length, C-terminal half (
700bp), and N terminal half (700bp).
【在 m*****n 的大作中提到】
: 自己做不行吗?
: 也不是很难,关键是要probe设计的好。
show any signal while other probes from other lab works very well as
positive control. Do you have any suggestion for probe design?
The mRNA is 1.5kb. I desgined three probes, full length, C-terminal half (
700bp), and N terminal half (700bp).
【在 m*****n 的大作中提到】
: 自己做不行吗?
: 也不是很难,关键是要probe设计的好。
k*n
8 楼
你可以试试invitrogen fish的kit,很好用
d*o
9 楼
700bp probe? 太大了
【在 s****9 的大作中提到】
: I am also doing in situ with DIG probe. None of the probes from my designs
: show any signal while other probes from other lab works very well as
: positive control. Do you have any suggestion for probe design?
: The mRNA is 1.5kb. I desgined three probes, full length, C-terminal half (
: 700bp), and N terminal half (700bp).
【在 s****9 的大作中提到】
: I am also doing in situ with DIG probe. None of the probes from my designs
: show any signal while other probes from other lab works very well as
: positive control. Do you have any suggestion for probe design?
: The mRNA is 1.5kb. I desgined three probes, full length, C-terminal half (
: 700bp), and N terminal half (700bp).
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