w*2
2 楼
真可能只是增加了LTE,和稍微换个chip。
苹果集中精力做ipanel (tv)
苹果集中精力做ipanel (tv)
y*a
3 楼
我构建了一个kinase promoter 的plasmid DNA,backbone 是PSB系统, 我的质粒浓度
是0.1ug/ul, 每次转化加1ug左右到50ul的农杆菌里。电击转化已经做了十几遍了,很
难得到菌落,偶尔能找到几个,划线后再挑单菌落做colony PCR还是不对。不知道是什
么原因?
是0.1ug/ul, 每次转化加1ug左右到50ul的农杆菌里。电击转化已经做了十几遍了,很
难得到菌落,偶尔能找到几个,划线后再挑单菌落做colony PCR还是不对。不知道是什
么原因?
c*b
6 楼
First, some questions for you:
1 Have you validated the plasmid by sequencing?
2 Have you tested the transformation efficiency of your agrobacteria?
3 What voltage have you been using? We used 1.44 kV normally.
4 Less likely, does your plasmid share the same antibiotic resistance as
your agro-bacterium?
Some suggestions:
try different strains. If you would like to do agro-infiltration, try C58C1
or GV3101. If you would like to generate transgenic plants, try LBA4404 or
GV3101.
Note:
C58C1 resists to Cif
GV3101 resists to Gentamycin
LBA4404 resists to Spec
【在 y*******a 的大作中提到】![](/moin_static193/solenoid/img/up.png)
: 我构建了一个kinase promoter 的plasmid DNA,backbone 是PSB系统, 我的质粒浓度
: 是0.1ug/ul, 每次转化加1ug左右到50ul的农杆菌里。电击转化已经做了十几遍了,很
: 难得到菌落,偶尔能找到几个,划线后再挑单菌落做colony PCR还是不对。不知道是什
: 么原因?
1 Have you validated the plasmid by sequencing?
2 Have you tested the transformation efficiency of your agrobacteria?
3 What voltage have you been using? We used 1.44 kV normally.
4 Less likely, does your plasmid share the same antibiotic resistance as
your agro-bacterium?
Some suggestions:
try different strains. If you would like to do agro-infiltration, try C58C1
or GV3101. If you would like to generate transgenic plants, try LBA4404 or
GV3101.
Note:
C58C1 resists to Cif
GV3101 resists to Gentamycin
LBA4404 resists to Spec
【在 y*******a 的大作中提到】
![](/moin_static193/solenoid/img/up.png)
: 我构建了一个kinase promoter 的plasmid DNA,backbone 是PSB系统, 我的质粒浓度
: 是0.1ug/ul, 每次转化加1ug左右到50ul的农杆菌里。电击转化已经做了十几遍了,很
: 难得到菌落,偶尔能找到几个,划线后再挑单菌落做colony PCR还是不对。不知道是什
: 么原因?
d*o
7 楼
找一个折叠键盘,没声!
l*e
10 楼
the new iPhone will definitely be revamped,
iphone is Apple's biggest revenue stream and lifeline.
The apple story is about the iPhone
Give up on iPhone to chase the so-called iPanel? Unless they are retard...
iphone is Apple's biggest revenue stream and lifeline.
The apple story is about the iPhone
Give up on iPhone to chase the so-called iPanel? Unless they are retard...
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