Brand New Method of DNA Sequencing# Biology - 生物学
w*u
1 楼
ba订票,改过一次,再改一次要交$70吗??谢谢
如果cancel的话,再重新订的话,要交几百块钱的税吗?
如果cancel的话,再重新订的话,要交几百块钱的税吗?
m*a
2 楼
注意到lenovo有一款要出来，但是现在市场上就有吗？要跟笔记本装的一样的win 7，体积要和ipad, HP touchpad一样小。毕竟要比app的话，谁能比得过windows呢？谁科普一下？
c*i
3 楼
Nicholas Wade, Decoding DNA With Semiconductors; New machine offers faster,
cheaper way to sequence genome. New York Times, July 21, 2011.
http://www.nytimes.com/2011/07/21/science/21genome.html
Excerpt in the window of print: It will take years to make sense of much of
teh data a novel technology reveals.
My comment:
(a) Techmnology. Ion Torrent, undated.
http://www.iontorrent.com/technology-scalability-simplicity-spe
Go to "How does it work?" in the left column. Clcik "Read more" and you
reach the text. By comparing the video clip, it is clear that the sequence
of the DNA for the last several nucleosides are 5'-T-G-A-C-T-T-3'.
(b) To be frank, I was not taught that a hydrogen ion would be released. The
following is what we are taught in biochemistry.
See the figure at page 866, in
Reginald Garrett and Charles M. Grisham. Biochemistry, fourth edition.
publisher: Mary Finch, 2010.
http://books.google.com/books?id=iGPsen3fSOIC&pg=
PA866&lpg=PA866&dq=%22dna+polymerase%22+elongation
+5'+biochemistry+%223'+oh%22&source=bl&ots=P9_lkwuZ03&sig
=Jl74E6oEjomoKueuR6F7XWyAO-Q&hl=en&ei=UHkoTuHyH8idgQeGlLRc&sa
=X&oi=book_result&ct=result&resnum=2&ved=0CBwQ6AEwAQ#v=onepage&q&f=false
("FIGURE 28.4 The chain elongation reaction catalyzed by DNA polymerase.The
3-OH carries out a nucleophilic attack on the -phosphoryl group of the
incoming dNTP to form a phosphoester bond, and PP is released. The
subsequent hydrolysis of PP, by inorganic pyrophosphatase renders teh
reaction effectively irreversible")
* dNTP is a short hand for deoxyNucleoside TriPhosphate. See the left panel
of the first, color graphic in
http://en.wikipedia.org/wiki/Nucleotide
* For laypersons, DNA elongation proceeds from 5' to 3'. The the phosphate of
the incoming dNTP would combine with the hydroxyl (OH) of 3' end.
cheaper way to sequence genome. New York Times, July 21, 2011.
http://www.nytimes.com/2011/07/21/science/21genome.html
Excerpt in the window of print: It will take years to make sense of much of
teh data a novel technology reveals.
My comment:
(a) Techmnology. Ion Torrent, undated.
http://www.iontorrent.com/technology-scalability-simplicity-spe
Go to "How does it work?" in the left column. Clcik "Read more" and you
reach the text. By comparing the video clip, it is clear that the sequence
of the DNA for the last several nucleosides are 5'-T-G-A-C-T-T-3'.
(b) To be frank, I was not taught that a hydrogen ion would be released. The
following is what we are taught in biochemistry.
See the figure at page 866, in
Reginald Garrett and Charles M. Grisham. Biochemistry, fourth edition.
publisher: Mary Finch, 2010.
http://books.google.com/books?id=iGPsen3fSOIC&pg=
PA866&lpg=PA866&dq=%22dna+polymerase%22+elongation
+5'+biochemistry+%223'+oh%22&source=bl&ots=P9_lkwuZ03&sig
=Jl74E6oEjomoKueuR6F7XWyAO-Q&hl=en&ei=UHkoTuHyH8idgQeGlLRc&sa
=X&oi=book_result&ct=result&resnum=2&ved=0CBwQ6AEwAQ#v=onepage&q&f=false
("FIGURE 28.4 The chain elongation reaction catalyzed by DNA polymerase.The
3-OH carries out a nucleophilic attack on the -phosphoryl group of the
incoming dNTP to form a phosphoester bond, and PP is released. The
subsequent hydrolysis of PP, by inorganic pyrophosphatase renders teh
reaction effectively irreversible")
* dNTP is a short hand for deoxyNucleoside TriPhosphate. See the left panel
of the first, color graphic in
http://en.wikipedia.org/wiki/Nucleotide
* For laypersons, DNA elongation proceeds from 5' to 3'. The the phosphate of
the incoming dNTP would combine with the hydroxyl (OH) of 3' end.
a*o
4 楼
If you cancel it online before ticketing, there is no extra charge.
Your miles will be redeposited almost instantly. But your booking fee
will not be refunded.
I never make a second change. But it does not hurt to try to make one.
【在 w**********u 的大作中提到】
: ba订票,改过一次,再改一次要交$70吗??谢谢
: 如果cancel的话,再重新订的话,要交几百块钱的税吗?
Your miles will be redeposited almost instantly. But your booking fee
will not be refunded.
I never make a second change. But it does not hurt to try to make one.
【在 w**********u 的大作中提到】
: ba订票,改过一次,再改一次要交$70吗??谢谢
: 如果cancel的话,再重新订的话,要交几百块钱的税吗?
N*w
5 楼
ASUS 有个 slate
还有几个厂有
反正比较耗电
，体积要和ipad, HP touchpad一样小。毕竟要比app的话，谁能比得过windows呢？谁
科普一下？
【在 m*****a 的大作中提到】
: 注意到lenovo有一款要出来，但是现在市场上就有吗？要跟笔记本装的一样的win 7，体积要和ipad, HP touchpad一样小。毕竟要比app的话，谁能比得过windows呢？谁科普一下？
还有几个厂有
反正比较耗电
，体积要和ipad, HP touchpad一样小。毕竟要比app的话，谁能比得过windows呢？谁
科普一下？
【在 m*****a 的大作中提到】
: 注意到lenovo有一款要出来，但是现在市场上就有吗？要跟笔记本装的一样的win 7，体积要和ipad, HP touchpad一样小。毕竟要比app的话，谁能比得过windows呢？谁科普一下？
w*e
6 楼
ion torrent 不新了吧？throughput还是低了点。
,
of
【在 c**i 的大作中提到】
: Nicholas Wade, Decoding DNA With Semiconductors; New machine offers faster,
: cheaper way to sequence genome. New York Times, July 21, 2011.
: http://www.nytimes.com/2011/07/21/science/21genome.html
: Excerpt in the window of print: It will take years to make sense of much of
: teh data a novel technology reveals.
: My comment:
: (a) Techmnology. Ion Torrent, undated.
: http://www.iontorrent.com/technology-scalability-simplicity-spe
: Go to "How does it work?" in the left column. Clcik "Read more" and you
: reach the text. By comparing the video clip, it is clear that the sequence
,
of
【在 c**i 的大作中提到】
: Nicholas Wade, Decoding DNA With Semiconductors; New machine offers faster,
: cheaper way to sequence genome. New York Times, July 21, 2011.
: http://www.nytimes.com/2011/07/21/science/21genome.html
: Excerpt in the window of print: It will take years to make sense of much of
: teh data a novel technology reveals.
: My comment:
: (a) Techmnology. Ion Torrent, undated.
: http://www.iontorrent.com/technology-scalability-simplicity-spe
: Go to "How does it work?" in the left column. Clcik "Read more" and you
: reach the text. By comparing the video clip, it is clear that the sequence
d*a
7 楼
acer w500
dell有一款屏幕能翻转的
，体积要和ipad, HP touchpad一样小。毕竟要比app的话，谁能比得过windows呢？谁
科普一下？
【在 m*****a 的大作中提到】
: 注意到lenovo有一款要出来，但是现在市场上就有吗？要跟笔记本装的一样的win 7，体积要和ipad, HP touchpad一样小。毕竟要比app的话，谁能比得过windows呢？谁科普一下？
dell有一款屏幕能翻转的
，体积要和ipad, HP touchpad一样小。毕竟要比app的话，谁能比得过windows呢？谁
科普一下？
【在 m*****a 的大作中提到】
: 注意到lenovo有一款要出来，但是现在市场上就有吗？要跟笔记本装的一样的win 7，体积要和ipad, HP touchpad一样小。毕竟要比app的话，谁能比得过windows呢？谁科普一下？
s*s
8 楼
这个版上都讨论了一年多了
【在 w******e 的大作中提到】
: ion torrent 不新了吧？throughput还是低了点。
:
: ,
: of
【在 w******e 的大作中提到】
: ion torrent 不新了吧？throughput还是低了点。
:
: ,
: of
N*w
9 楼
ASUS EEE Slate 阿
【在 d******a 的大作中提到】
: acer w500
: dell有一款屏幕能翻转的
:
: ，体积要和ipad, HP touchpad一样小。毕竟要比app的话，谁能比得过windows呢？谁
: 科普一下？
【在 d******a 的大作中提到】
: acer w500
: dell有一款屏幕能翻转的
:
: ，体积要和ipad, HP touchpad一样小。毕竟要比app的话，谁能比得过windows呢？谁
: 科普一下？
m*T
10 楼
主要是第一篇介绍ION技术的paper刚在nature上发表了。公司的marketing自然不会放
过这么好的机会
过这么好的机会
f*e
11 楼
这方法通量不行，library prep 和 solid 一样 ”好“。
e*e
12 楼
This is a more insightful post if you care about the study:
How accurate is the new Ion Torrent genome, really?
http://m.wired.com/wiredscience/2011/07/how-accurate-is-the-new
,
of
【在 c**i 的大作中提到】
: Nicholas Wade, Decoding DNA With Semiconductors; New machine offers faster,
: cheaper way to sequence genome. New York Times, July 21, 2011.
: http://www.nytimes.com/2011/07/21/science/21genome.html
: Excerpt in the window of print: It will take years to make sense of much of
: teh data a novel technology reveals.
: My comment:
: (a) Techmnology. Ion Torrent, undated.
: http://www.iontorrent.com/technology-scalability-simplicity-spe
: Go to "How does it work?" in the left column. Clcik "Read more" and you
: reach the text. By comparing the video clip, it is clear that the sequence
How accurate is the new Ion Torrent genome, really?
http://m.wired.com/wiredscience/2011/07/how-accurate-is-the-new
,
of
【在 c**i 的大作中提到】
: Nicholas Wade, Decoding DNA With Semiconductors; New machine offers faster,
: cheaper way to sequence genome. New York Times, July 21, 2011.
: http://www.nytimes.com/2011/07/21/science/21genome.html
: Excerpt in the window of print: It will take years to make sense of much of
: teh data a novel technology reveals.
: My comment:
: (a) Techmnology. Ion Torrent, undated.
: http://www.iontorrent.com/technology-scalability-simplicity-spe
: Go to "How does it work?" in the left column. Clcik "Read more" and you
: reach the text. By comparing the video clip, it is clear that the sequence
p*r
13 楼
大家对Pacific bio和Complete Genome的技术怎么看？
f*e
14 楼
请爬文
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