C*Y
2 楼
I am trying to generate cell line with inducible knockdown. I used Tet-pLKO
-puro, in which a puromycin resistant gene is placed downstream of pGK-TetR-
IRES. The problem I am having now is that the expression level of puromycin
resistant gene was so low that all the cells were killed by puromycin. I
heard that the single vector Tet-induicible system selling by Clontech is
not very good either. Have anyone found any system satisfactory?
Thanks.
-puro, in which a puromycin resistant gene is placed downstream of pGK-TetR-
IRES. The problem I am having now is that the expression level of puromycin
resistant gene was so low that all the cells were killed by puromycin. I
heard that the single vector Tet-induicible system selling by Clontech is
not very good either. Have anyone found any system satisfactory?
Thanks.
s*l
3 楼
NCT已经走出困境，发dividend时会冲10.
【在 c******e 的大作中提到】
【在 c******e 的大作中提到】
b*8
4 楼
Why did you say the single vector Tet-induicible system selling by Clontech
is not very good? In contrast, I read many papers in which the authors used
the syetem to devolope inducible kd stable cell line and conduct in vivo&
vitro study. Below are some of them,
1)THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 283, NO. 29, pp. 20383–20396,
July 18, 2008
2)J Clin Invest. 2010;120(12):4289–4302. doi:10.1172/JCI42015.
is not very good? In contrast, I read many papers in which the authors used
the syetem to devolope inducible kd stable cell line and conduct in vivo&
vitro study. Below are some of them,
1)THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 283, NO. 29, pp. 20383–20396,
July 18, 2008
2)J Clin Invest. 2010;120(12):4289–4302. doi:10.1172/JCI42015.
a*g
5 楼
怎么翻了5倍？我看错了吗
【在 c******e 的大作中提到】
【在 c******e 的大作中提到】
C*Y
6 楼
Thanks for the response. I found the information here.
http://www.protocol-online.org/biology-forums-2/posts/7444.html
Clontech
used
【在 b**********8 的大作中提到】
: Why did you say the single vector Tet-induicible system selling by Clontech
: is not very good? In contrast, I read many papers in which the authors used
: the syetem to devolope inducible kd stable cell line and conduct in vivo&
: vitro study. Below are some of them,
: 1)THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 283, NO. 29, pp. 20383–20396,
: July 18, 2008
: 2)J Clin Invest. 2010;120(12):4289–4302. doi:10.1172/JCI42015.
http://www.protocol-online.org/biology-forums-2/posts/7444.html
Clontech
used
【在 b**********8 的大作中提到】
: Why did you say the single vector Tet-induicible system selling by Clontech
: is not very good? In contrast, I read many papers in which the authors used
: the syetem to devolope inducible kd stable cell line and conduct in vivo&
: vitro study. Below are some of them,
: 1)THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 283, NO. 29, pp. 20383–20396,
: July 18, 2008
: 2)J Clin Invest. 2010;120(12):4289–4302. doi:10.1172/JCI42015.
q*u
7 楼
骨灰级大牛回到古板了，欢迎！欢迎！
【在 c******e 的大作中提到】
【在 c******e 的大作中提到】
s*f
8 楼
这坟挖的
l*j
9 楼
这是来自未来的大牛?还是预测Nov,21,2010会涨５倍?
【在 c******e 的大作中提到】
【在 c******e 的大作中提到】
s*l
10 楼
2008,赫赫。
怎么翻了5倍？我看错了吗
【在 a****g 的大作中提到】
: 怎么翻了5倍？我看错了吗
怎么翻了5倍？我看错了吗
【在 a****g 的大作中提到】
: 怎么翻了5倍？我看错了吗
w*s
11 楼
Newcastle investment
just ER-ed
no strong outlook
just ER-ed
no strong outlook
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