Markers for G2 and M phase of cell cycle? - 未名空间MITBBS历史存档

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Markers for G2 and M phase of cell cycle?

Markers for G2 and M phase of cell cycle?# Biology - 生物学

s*m2012-01-09 08:01

1 楼

Hello Everyone,
Anybody studied cell cycle here? Is there any assays or markers to
distinguish G2 phase from M phase easily?
Thank you so much!

m*52012-01-09 08:01

2 楼

phorshpor Histon 3 is a common marker for mitosis phase
you can do a westernblot to find out
IHC also works

b*02012-01-09 08:01

3 楼

you can directly tell if the cell is in M phase by looking at the chromosome
arrangement. Cells in M phase tend to have no nuclear membrane and
condensed chromsomes are ready to be aligned at the plate.
A good marker for cells from last S to G2 phase is CEP-F antibody.

【在 s****m 的大作中提到】

: Hello Everyone,
: Anybody studied cell cycle here? Is there any assays or markers to
: distinguish G2 phase from M phase easily?
: Thank you so much!

s*m2012-01-09 08:01

4 楼

thanks, I just check the abcam, where it has the human Anti-CENPF antibody,
is there any mouse or goat anti-CENPF antibody?
Thanks again

chromosome

【在 b*******0 的大作中提到】

: you can directly tell if the cell is in M phase by looking at the chromosome
: arrangement. Cells in M phase tend to have no nuclear membrane and
: condensed chromsomes are ready to be aligned at the plate.
: A good marker for cells from last S to G2 phase is CEP-F antibody.