h*e
2 楼
Just wondering if there is a way to quantify a specific gene expression in
mixed population of cells.
For example:
Each sample has x number of cells from normal tissue mixed with y number of
cancer cells. x and y are unknown.
How to compare some specific gene expression profiles (those genes only
expressed in cancer cells) among different
samples.
Any reference?
mixed population of cells.
For example:
Each sample has x number of cells from normal tissue mixed with y number of
cancer cells. x and y are unknown.
How to compare some specific gene expression profiles (those genes only
expressed in cancer cells) among different
samples.
Any reference?
w*r
4 楼
flow cytometry? use some marker to label cancer cell
of
【在 h***e 的大作中提到】
: Just wondering if there is a way to quantify a specific gene expression in
: mixed population of cells.
: For example:
: Each sample has x number of cells from normal tissue mixed with y number of
: cancer cells. x and y are unknown.
: How to compare some specific gene expression profiles (those genes only
: expressed in cancer cells) among different
: samples.
: Any reference?
of
【在 h***e 的大作中提到】
: Just wondering if there is a way to quantify a specific gene expression in
: mixed population of cells.
: For example:
: Each sample has x number of cells from normal tissue mixed with y number of
: cancer cells. x and y are unknown.
: How to compare some specific gene expression profiles (those genes only
: expressed in cancer cells) among different
: samples.
: Any reference?
l*y
6 楼
if you have controls.
of
【在 h***e 的大作中提到】
: Just wondering if there is a way to quantify a specific gene expression in
: mixed population of cells.
: For example:
: Each sample has x number of cells from normal tissue mixed with y number of
: cancer cells. x and y are unknown.
: How to compare some specific gene expression profiles (those genes only
: expressed in cancer cells) among different
: samples.
: Any reference?
of
【在 h***e 的大作中提到】
: Just wondering if there is a way to quantify a specific gene expression in
: mixed population of cells.
: For example:
: Each sample has x number of cells from normal tissue mixed with y number of
: cancer cells. x and y are unknown.
: How to compare some specific gene expression profiles (those genes only
: expressed in cancer cells) among different
: samples.
: Any reference?
k*4
9 楼
ppi也高
★ 发自iPhone App: ChineseWeb 8.7
★ 发自iPhone App: ChineseWeb 8.7
c*t
13 楼
只差100刀啊。
h*e
14 楼
Many people normalize the expression to the housekeeping genes expression;
but what if I don't know how many normal/cancer cells, considering both
normal/cancer cell contribute to the housekeeping gene expression?
heterogeneous?
【在 l***y 的大作中提到】
: How about using normal cells as the control? Are cancer cells heterogeneous?
: if so, you still cannot get rid of the so-called "volume effects".
but what if I don't know how many normal/cancer cells, considering both
normal/cancer cell contribute to the housekeeping gene expression?
heterogeneous?
【在 l***y 的大作中提到】
: How about using normal cells as the control? Are cancer cells heterogeneous?
: if so, you still cannot get rid of the so-called "volume effects".
l*u
16 楼
laser capture microdissections to capture pure tumor cell population.
of
【在 h***e 的大作中提到】
: Just wondering if there is a way to quantify a specific gene expression in
: mixed population of cells.
: For example:
: Each sample has x number of cells from normal tissue mixed with y number of
: cancer cells. x and y are unknown.
: How to compare some specific gene expression profiles (those genes only
: expressed in cancer cells) among different
: samples.
: Any reference?
of
【在 h***e 的大作中提到】
: Just wondering if there is a way to quantify a specific gene expression in
: mixed population of cells.
: For example:
: Each sample has x number of cells from normal tissue mixed with y number of
: cancer cells. x and y are unknown.
: How to compare some specific gene expression profiles (those genes only
: expressed in cancer cells) among different
: samples.
: Any reference?
l*y
20 楼
Actually I am working on an approach to address similar issues.
A friend of mine, who was a postdoc in my PhD lab, worked on FTIR-based
polymer composition assay. He developed a very simple spectrum-based math
method, and the performance was amazing.
I am applying the same idea and developing a spectrum mixture model to
decouple cell phenotypes and reveal the GE features of each phenotype. The
major limitation is the volume effects, and I have worked out a workaround
method.
If reviewers buy the idea, I will let you know.
【在 h***e 的大作中提到】
: Many people normalize the expression to the housekeeping genes expression;
: but what if I don't know how many normal/cancer cells, considering both
: normal/cancer cell contribute to the housekeeping gene expression?
:
: heterogeneous?
A friend of mine, who was a postdoc in my PhD lab, worked on FTIR-based
polymer composition assay. He developed a very simple spectrum-based math
method, and the performance was amazing.
I am applying the same idea and developing a spectrum mixture model to
decouple cell phenotypes and reveal the GE features of each phenotype. The
major limitation is the volume effects, and I have worked out a workaround
method.
If reviewers buy the idea, I will let you know.
【在 h***e 的大作中提到】
: Many people normalize the expression to the housekeeping genes expression;
: but what if I don't know how many normal/cancer cells, considering both
: normal/cancer cell contribute to the housekeeping gene expression?
:
: heterogeneous?
f*w
26 楼
b*r
29 楼
为了让眼睛更舒服呗
j*f
30 楼
因为手大。note无压力
d*6
31 楼
看个人需要 别跟风
我还是希望手机能放在裤子口袋里...所以定了6
我还是希望手机能放在裤子口袋里...所以定了6
l*z
32 楼
对,俺officemate 摸了6+后果断的定了6。人可是185的个头,就是觉得手机就不应该
太大
太大
k*4
36 楼
出门都带包包,表示5.5无压力。不过最喜欢的尺寸其实是s5的5.1寸。
★ 发自iPhone App: ChineseWeb 8.7
★ 发自iPhone App: ChineseWeb 8.7
r*p
39 楼
OIS,在室内照相night and day的差别。用过Lumia 920以上的就再难接受没有OIS的手
机了。
机了。
相关阅读
国内的中医专业都学些啥?屠奶奶这个月23号有演讲俺老板说中医这套东西跟中世纪欧洲的医学体系差不多ATG trap中,atg和后面的基因最远能隔多远啊?zz 思考留给科学,实验外包出去 (果壳)What is 80-kb resolution有没有用过Vasa Cre的前辈,望指点.国家自然科学奖今年再次出现空缺 (转载)请教各位达人,top2的教授职位值不值得归?老杨在复旦谈金力薄厚位置选择有人去CSHL的oligonucleotide therapeutics meeting吗?Paper help!求论文搞CS的过来挺一下中医吧朱镕基的儿子朱云也护专业阿青蒿素是唯一进英文药理教科书的国药求助,看看这是什么情况的老鼠...从中医的药物服用方法想到药物的提取方法。强烈祝贺屠屠呦呦获得拉斯克奖