h*e
2 楼
Just wondering if there is a way to quantify a specific gene expression in
mixed population of cells.
For example:
Each sample has x number of cells from normal tissue mixed with y number of
cancer cells. x and y are unknown.
How to compare some specific gene expression profiles (those genes only
expressed in cancer cells) among different
samples.
Any reference?
mixed population of cells.
For example:
Each sample has x number of cells from normal tissue mixed with y number of
cancer cells. x and y are unknown.
How to compare some specific gene expression profiles (those genes only
expressed in cancer cells) among different
samples.
Any reference?
w*r
4 楼
flow cytometry? use some marker to label cancer cell
of
【在 h***e 的大作中提到】
: Just wondering if there is a way to quantify a specific gene expression in
: mixed population of cells.
: For example:
: Each sample has x number of cells from normal tissue mixed with y number of
: cancer cells. x and y are unknown.
: How to compare some specific gene expression profiles (those genes only
: expressed in cancer cells) among different
: samples.
: Any reference?
of
【在 h***e 的大作中提到】
: Just wondering if there is a way to quantify a specific gene expression in
: mixed population of cells.
: For example:
: Each sample has x number of cells from normal tissue mixed with y number of
: cancer cells. x and y are unknown.
: How to compare some specific gene expression profiles (those genes only
: expressed in cancer cells) among different
: samples.
: Any reference?
l*y
6 楼
if you have controls.
of
【在 h***e 的大作中提到】
: Just wondering if there is a way to quantify a specific gene expression in
: mixed population of cells.
: For example:
: Each sample has x number of cells from normal tissue mixed with y number of
: cancer cells. x and y are unknown.
: How to compare some specific gene expression profiles (those genes only
: expressed in cancer cells) among different
: samples.
: Any reference?
of
【在 h***e 的大作中提到】
: Just wondering if there is a way to quantify a specific gene expression in
: mixed population of cells.
: For example:
: Each sample has x number of cells from normal tissue mixed with y number of
: cancer cells. x and y are unknown.
: How to compare some specific gene expression profiles (those genes only
: expressed in cancer cells) among different
: samples.
: Any reference?
k*4
9 楼
ppi也高
★ 发自iPhone App: ChineseWeb 8.7
★ 发自iPhone App: ChineseWeb 8.7
c*t
13 楼
只差100刀啊。
h*e
14 楼
Many people normalize the expression to the housekeeping genes expression;
but what if I don't know how many normal/cancer cells, considering both
normal/cancer cell contribute to the housekeeping gene expression?
heterogeneous?
【在 l***y 的大作中提到】
: How about using normal cells as the control? Are cancer cells heterogeneous?
: if so, you still cannot get rid of the so-called "volume effects".
but what if I don't know how many normal/cancer cells, considering both
normal/cancer cell contribute to the housekeeping gene expression?
heterogeneous?
【在 l***y 的大作中提到】
: How about using normal cells as the control? Are cancer cells heterogeneous?
: if so, you still cannot get rid of the so-called "volume effects".
l*u
16 楼
laser capture microdissections to capture pure tumor cell population.
of
【在 h***e 的大作中提到】
: Just wondering if there is a way to quantify a specific gene expression in
: mixed population of cells.
: For example:
: Each sample has x number of cells from normal tissue mixed with y number of
: cancer cells. x and y are unknown.
: How to compare some specific gene expression profiles (those genes only
: expressed in cancer cells) among different
: samples.
: Any reference?
of
【在 h***e 的大作中提到】
: Just wondering if there is a way to quantify a specific gene expression in
: mixed population of cells.
: For example:
: Each sample has x number of cells from normal tissue mixed with y number of
: cancer cells. x and y are unknown.
: How to compare some specific gene expression profiles (those genes only
: expressed in cancer cells) among different
: samples.
: Any reference?
l*y
20 楼
Actually I am working on an approach to address similar issues.
A friend of mine, who was a postdoc in my PhD lab, worked on FTIR-based
polymer composition assay. He developed a very simple spectrum-based math
method, and the performance was amazing.
I am applying the same idea and developing a spectrum mixture model to
decouple cell phenotypes and reveal the GE features of each phenotype. The
major limitation is the volume effects, and I have worked out a workaround
method.
If reviewers buy the idea, I will let you know.
【在 h***e 的大作中提到】
: Many people normalize the expression to the housekeeping genes expression;
: but what if I don't know how many normal/cancer cells, considering both
: normal/cancer cell contribute to the housekeeping gene expression?
:
: heterogeneous?
A friend of mine, who was a postdoc in my PhD lab, worked on FTIR-based
polymer composition assay. He developed a very simple spectrum-based math
method, and the performance was amazing.
I am applying the same idea and developing a spectrum mixture model to
decouple cell phenotypes and reveal the GE features of each phenotype. The
major limitation is the volume effects, and I have worked out a workaround
method.
If reviewers buy the idea, I will let you know.
【在 h***e 的大作中提到】
: Many people normalize the expression to the housekeeping genes expression;
: but what if I don't know how many normal/cancer cells, considering both
: normal/cancer cell contribute to the housekeeping gene expression?
:
: heterogeneous?
f*w
26 楼
b*r
29 楼
为了让眼睛更舒服呗
j*f
30 楼
因为手大。note无压力
d*6
31 楼
看个人需要 别跟风
我还是希望手机能放在裤子口袋里...所以定了6
我还是希望手机能放在裤子口袋里...所以定了6
l*z
32 楼
对,俺officemate 摸了6+后果断的定了6。人可是185的个头,就是觉得手机就不应该
太大
太大
k*4
36 楼
出门都带包包,表示5.5无压力。不过最喜欢的尺寸其实是s5的5.1寸。
★ 发自iPhone App: ChineseWeb 8.7
★ 发自iPhone App: ChineseWeb 8.7
r*p
39 楼
OIS,在室内照相night and day的差别。用过Lumia 920以上的就再难接受没有OIS的手
机了。
机了。
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