a quick question about OriGene plasmid product!! I am devastated about it - 未名空间MITBBS历史存档

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a quick question about OriGene plasmid product!! I am devastated about it

a quick question about OriGene plasmid product!! I am devastated about it# Biology - 生物学

t*n2013-03-11 07:03

1 楼

俺本来不会做龙虾的，实在抵不过3.99刀/磅的便宜诱惑。。。
好在网络发达，再没做过的东西放狗搜一下，就那啥了。。。
不废话啦，看图片吧，前后两顿的。。。
葱爆龙虾：
1，龙虾放尿、洗净，切块，加盐、胡椒面、姜粉、料酒盐10来分钟，撒点淀粉，热油
锅，过油，捞起来
2，另起一锅，加少量油，下姜片、葱段、洋葱呛锅，然后下龙虾，2～3分钟起锅～
麻辣龙虾：
第一步同上
2，另起一锅，加少量油，下豆瓣酱、干辣椒花椒呛锅，然后下龙虾，2～3分钟起锅～
清煮：
龙虾放尿、洗净，水烧开，龙虾扔进去煮20分钟，起锅开吃～～～
over

m*52013-03-11 07:03

2 楼

Did anyone here used plasmid or cDNA clones from OriGene?
is their product reliable?
Moreover, in many of their plasmid they denote the plasmid are not
replicable by regular DNA purification method, is this possible? Looks to me
they are just trying to get customers to buy the plasmid from them instead
of purifying the plasmid indefinitely in their own bench
I attached their 'explanation below'
;Due to the inherent nature of this plasmid, standard methods to replicate
additional amounts of DNA in E. coli are highly likely to result in
mutations and/or rearrangements. Therefore, OriGene does not guarantee the
capability to replicate this plasmid DNA. Additional amounts of DNA can be
purchased from OriGene with batch-specific, full-sequence verification at a
reduced cost. Please contact our customer care team at [email protected]
com or by calling 301.340.3188 option 3 for pricing and delivery.;

j*u2013-03-11 07:03

3 楼

3.99?!!什么地方这么便宜？！

【在 t*****n 的大作中提到】

: 俺本来不会做龙虾的，实在抵不过3.99刀/磅的便宜诱惑。。。
: 好在网络发达，再没做过的东西放狗搜一下，就那啥了。。。
: 不废话啦，看图片吧，前后两顿的。。。
: 葱爆龙虾：
: 1，龙虾放尿、洗净，切块，加盐、胡椒面、姜粉、料酒盐10来分钟，撒点淀粉，热油
: 锅，过油，捞起来
: 2，另起一锅，加少量油，下姜片、葱段、洋葱呛锅，然后下龙虾，2～3分钟起锅～
: 麻辣龙虾：
: 第一步同上
: 2，另起一锅，加少量油，下豆瓣酱、干辣椒花椒呛锅，然后下龙虾，2～3分钟起锅～