分享一个有意思的文章# Biology - 生物学
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版上火药味好浓啊，大家看看文章散散心吧。
新出的文章认为actin 的总体动态以及相关的serum response factor 是一个生理时钟。
Cell. 2013 Jan 31;152(3):492-503. doi: 10.1016/j.cell.2012.12.027.
Blood-borne circadian signal stimulates daily oscillations in actin dynamics
and SRF activity.
Gerber A, Esnault C, Aubert G, Treisman R, Pralong F, Schibler U.
Source
Department of Molecular Biology, Sciences III, University of Geneva, and
National Centre of Competence in Research Frontiers in Genetics, 1211 Geneva
, Switzerland.
Abstract
In peripheral tissues circadian gene expression can be driven either by
local oscillators or by cyclic systemic cues controlled by the master clock
in the brain's suprachiasmatic nucleus. In the latter case, systemic signals
can activate immediate early transcription factors (IETFs) and thereby
control rhythmic transcription. In order to identify IETFs induced by
diurnal blood-borne signals, we developed an unbiased experimental strategy,
dubbed Synthetic TAndem Repeat PROMoter (STAR-PROM) screening. This
technique relies on the observation that most transcription factor binding
sites exist at a relatively high frequency in random DNA sequences. Using
STAR-PROM we identified serum response factor (SRF) as an IETF responding to
oscillating signaling proteins present in human and rodent sera. Our data
suggest that in mouse liver SRF is regulated via dramatic diurnal changes of
actin dynamics, leading to the rhythmic translocation of the SRF
coactivator Myocardin-related transcription factor-B (MRTF-B) into the
nucleus.
新出的文章认为actin 的总体动态以及相关的serum response factor 是一个生理时钟。
Cell. 2013 Jan 31;152(3):492-503. doi: 10.1016/j.cell.2012.12.027.
Blood-borne circadian signal stimulates daily oscillations in actin dynamics
and SRF activity.
Gerber A, Esnault C, Aubert G, Treisman R, Pralong F, Schibler U.
Source
Department of Molecular Biology, Sciences III, University of Geneva, and
National Centre of Competence in Research Frontiers in Genetics, 1211 Geneva
, Switzerland.
Abstract
In peripheral tissues circadian gene expression can be driven either by
local oscillators or by cyclic systemic cues controlled by the master clock
in the brain's suprachiasmatic nucleus. In the latter case, systemic signals
can activate immediate early transcription factors (IETFs) and thereby
control rhythmic transcription. In order to identify IETFs induced by
diurnal blood-borne signals, we developed an unbiased experimental strategy,
dubbed Synthetic TAndem Repeat PROMoter (STAR-PROM) screening. This
technique relies on the observation that most transcription factor binding
sites exist at a relatively high frequency in random DNA sequences. Using
STAR-PROM we identified serum response factor (SRF) as an IETF responding to
oscillating signaling proteins present in human and rodent sera. Our data
suggest that in mouse liver SRF is regulated via dramatic diurnal changes of
actin dynamics, leading to the rhythmic translocation of the SRF
coactivator Myocardin-related transcription factor-B (MRTF-B) into the
nucleus.
