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CAR-T cell的疑问

CAR-T cell的疑问# Biology - 生物学

n*y2013-05-22 07:05

1 楼

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d*r2013-05-22 07:05

2 楼

http://www.ncbi.nlm.nih.gov/pubmed/21832238
见附图. 我的疑问是: T cell 都被CD3/CD28 beads包围了, 这时候来做lentivirus
transduction, 不是明显减少了很多细胞的表面来供viral vector进攻吗? 这不会影响
transduction efficiency吗?

l*n2013-05-22 07:05

3 楼

谢谢LZ。但是没明白那10% mover coupon怎么搞啊？

S*s2013-05-22 07:05

4 楼

The bead should be washed out before putting into bag.

【在 d********r 的大作中提到】

: http://www.ncbi.nlm.nih.gov/pubmed/21832238
: 见附图. 我的疑问是: T cell 都被CD3/CD28 beads包围了, 这时候来做lentivirus
: transduction, 不是明显减少了很多细胞的表面来供viral vector进攻吗? 这不会影响
: transduction efficiency吗?

d*r2013-05-22 07:05

5 楼

are you sure? 见附图, harvest day 才remove beads啊.

【在 S*****s 的大作中提到】

: The bead should be washed out before putting into bag.

d*r2013-05-22 07:05

6 楼

还是这篇文章, 他们怎么把successfully transfected cells 和 non-transfected
cells 分开的啊?

【在 d********r 的大作中提到】

l*72013-05-22 07:05

7 楼

不用分，临床已经够用的了。这玩艺在体内可以扩增。不需要太高的转化效率。

d*p2013-05-22 07:05

8 楼

Beads are much smaller than T cells. 1:1 ratio is good enough to activate T
cells. I suggest you learn what T cell synapse is, if you don't have
immunology background.

d*r2013-05-22 07:05

9 楼

CD3/CD28 beads are 4.5 μm diameter, not too small. (see in the link below)
http://products.invitrogen.com/ivgn/product/11161D
Also I think they are using 3:1 ratio.
Some information suggests that transduction may happen better when the cells
are being activated, so they may want to have the beads there...
Not sure...

T

【在 d*p 的大作中提到】

: Beads are much smaller than T cells. 1:1 ratio is good enough to activate T
: cells. I suggest you learn what T cell synapse is, if you don't have
: immunology background.