d*n
2 楼
h1-b 明年九月就满六年了。I-140 是2010 年10月批的。现在有一个公司准备雇用我。
这个新的h1-b是3年的呢，还是10个月的呢？ 假如新h1-b只有十个月，到时候新的i-
140还没有下来，旧的i-140被revoke. h1-b是不是就无法延期了？
这个新的h1-b是3年的呢，还是10个月的呢？ 假如新h1-b只有十个月，到时候新的i-
140还没有下来，旧的i-140被revoke. h1-b是不是就无法延期了？
n*m
3 楼
Immunology Retraction Exposes Misconduct
07/10/2013 Andrew S. Wiecek
Nature retracts a 1994 paper after the first author was found to have "most
likely" committed research misconduct. Learn more...
Last week, Nature retracted a highly-cited paper published in 1994 because
the authors were unable to reproduce the results, according to the
retraction notice (1). But the real reason behind the retraction might have
more to do with the first author’s scientific misconduct.
Nature has retracted a 1994 paper because first author Karel Bezouska was
found to have "most likely" committed research misconduct. Source: https://
www.natur.cuni.cz
In the 1994 paper (2), first author Karel Bezouska and colleagues from
Northwick Park Hospital in the UK, the Charles University of Prague, and the
Institute of Microbiology of the Czech Academy of Sciences reported that
they had identified carbohydrate oligosaccharide ligands that bound strongly
to NKR-P1, a membrane protein on natural killer cells. Natural killer cells
are part of the immune system. Their function is to identify and mark
virally infected cells and tumor cells for death.
Some tumor cells, however, are resistant to natural killer cells, allowing
them to evade targeted cell destruction. So when the researchers claimed in
their paper that incubating resistant tumor cells with the oligosaccharide
ligands they had identified made the cells vulnerable to elimination by
natural killer cells, it was considered a breakthrough.
“The findings were indeed considered a big at the time,” lead author Ten
Feizi, whose lab Bezouska was working in when he wrote the 1994 paper, said
in an email to BioTechniques. Feizi is now a professor at Imperial College
London.
Since publication, the paper has been cited 257 times since publication,
according to Thomson Reuters Web of Knowledge. “It is most likely that many
of the citations were by scientists unaware of the irreproducibility. I say
this because I made a point of informing key people in the field about the
problem, and between us when refereeing papers citing the article, we
discouraged citation,” said Feizi.
Two years later, a correction to the paper was published in Nature (3). In
that notice, the researchers said that they were unable to reproduce the
results. As a result, the team was re-evaluating the experimental system,
which was set up in Prague by three of the study authors: Bezouska, Anna
Fiserova, and Miloslav Pospíšil. The team promised to publish the
results of their re-evaluation as soon as possible.
But it wasn’t until just last week, 19 years after the original article was
published, that the paper was finally retracted. And that retraction came
without the signatures of Bezouska, Lawson, Pospíšil (now deceased),
and another author who could not be found.
As it turns out, the retraction only came to pass because first author
Bezouska was found by an ethics committee at Charles University in January
2013 to have “most likely” committed scientific misconduct in the
experiments published in the 1994 Nature paper and in follow-up studies, as
reported by Retraction Watch. As a result, Bezouska was terminated from the
Charles University and the Institute of Microbiology. In their press
releases, the 2 organizations urged his coauthors to review the more than
100 peer-reviewed publications that he had published for invalid results.
Although the irreproducible data from the 1994 paper were mentioned in the
report, the most conclusive evidence of Bezouska’s misconduct came from
Professor Vladimir Kren and three students from his laboratory at the
Institute of Microbiology who, in 2012, suspected someone was manipulating
their NKR-P1 carbohydrate binding experiments. So they installed a camera to
monitor their samples overnight. The camera caught Bezouska red-handed,
showing him manipulating the samples to generate positive results.
“…Bezouska fabricated practically all results dealing with NKR-P1 and CD69
receptors,” said Kren in an email to BioTechniques. CD69 receptors are
another surface protein on NK cells. Bezouska claimed to have identified
high-affinity ligands for CD69 receptors in a 1995 Biochemical and
Biophysical Research Communications paper (4). “At present, we estimate
that there [are] some 20 papers contaminated by this misconduct, and these
papers have to be corrected or retracted.”
Although Feizi wanted to retract the 1994 paper when the correction was
published in 1996, Nature’s editorial policy at the time required that
retractions be accompanied by the signature of all authors, according to an
email that she wrote to Retraction Watch. Feizi, however, did publish two
studies in other journals that re-evaluated the results from the 1994 Nature
paper and the 1995 Biochemical and Biophysical Research Communications
paper, concluding that all those results were irreproducible (5-6).
In addition, Feizi published a correction in 1999 to another paper that she
co-authored with Bezouska in 1994 in the Journal of Biological Chemistry,
also on the binding of carbohydrates to NKR-P1 (7-8).
References
1. Bezouska, K., C.-T. Yuen, J. O/'Brien, R. A. Childs, W. Chai, A. M.
Lawson, K. Drbal, A. Fiserova, M. Pospisil, and T. Feizi. 2013. Retraction:
Oligosaccharide ligands for NKR-p1 protein activate NK cells and
cytotoxicity. Nature advance online publication(July).
2. Bezouska, K., C.-T. Yuen, J. O'Brien, R. A. Childs, W. Chai, A. M. Lawson
, K. Drbal, A. Fiserova, M. Posisil, and T. Feizi. 1994. Oligosaccharide
ligands for NKR-p1 protein activate NK cells and cytotoxicity. Nature 372(
6502):150-157.
3.Bezou[caron]ka, K., C.-T. Yuen, J. O'Brien, R. A. Childs, W. Chai, A. M.
Lawson, K. Drbal, A. Fis[caron]erova, M. PospIs[caron]il, and T. Feizi. 1996
. Oligosaccharide ligands for NKR-p1 protein activate NK cells and
cytotoxicity. Nature 380(6574):559.
4. Bezouska, K., A. Nepovím, O. Horváth, M. Pospísil, J. Hamann, and T.
Feizi. 1995. CD 69 antigen of human lymphocytes is a calcium-dependent
carbohydrate-binding protein. Biochemical and biophysical research
communications 208(1):68-74.
5. Kogelberg, H., A. M. Lawson, F. W. Muskett, R. A. Carruthers, and T.
Feizi. 2000. Expression in escherichia coli, folding in vitro, and
characterization of the carbohydrate recognition domain of the natural
killer cell receptor NKR-P1A. Protein Expression and Purification 20(1):10-
20.
6. Childs, R. A., C. Galustian, A. M. Lawson, G. Dougan, K. Benwell, G.
Frankel, and T. Feizi. 1999. Recombinant soluble human CD69 dimer produced
in escherichia coli: Reevaluation of saccharide binding. Biochemical and
Biophysical Research Communications 266(1):19-23.
7. Kogelberg, H., E. Montero, S. Bay, A. M. Lawson, and T. Feizi. 1999. Re-
evaluation of monosaccharide binding property of recombinant soluble
carbohydrate recognition domain of the natural killer cell receptor NKR-P1A.
Journal of Biological Chemistry 274(42):30335.
8. Bezouska, K., G. Vlahas, O. Horváth, G. Jinochová, A. Fiserová, R.
Giorda, W. H. Chambers, T. Feizi, and M. Pospísil. 1994. Rat natural killer
cell antigen, NKR-p1, related to c-type animal lectins is a carbohydrate-
binding protein. Journal of Biological Chemistry 269(24):16945-16952.
07/10/2013 Andrew S. Wiecek
Nature retracts a 1994 paper after the first author was found to have "most
likely" committed research misconduct. Learn more...
Last week, Nature retracted a highly-cited paper published in 1994 because
the authors were unable to reproduce the results, according to the
retraction notice (1). But the real reason behind the retraction might have
more to do with the first author’s scientific misconduct.
Nature has retracted a 1994 paper because first author Karel Bezouska was
found to have "most likely" committed research misconduct. Source: https://
www.natur.cuni.cz
In the 1994 paper (2), first author Karel Bezouska and colleagues from
Northwick Park Hospital in the UK, the Charles University of Prague, and the
Institute of Microbiology of the Czech Academy of Sciences reported that
they had identified carbohydrate oligosaccharide ligands that bound strongly
to NKR-P1, a membrane protein on natural killer cells. Natural killer cells
are part of the immune system. Their function is to identify and mark
virally infected cells and tumor cells for death.
Some tumor cells, however, are resistant to natural killer cells, allowing
them to evade targeted cell destruction. So when the researchers claimed in
their paper that incubating resistant tumor cells with the oligosaccharide
ligands they had identified made the cells vulnerable to elimination by
natural killer cells, it was considered a breakthrough.
“The findings were indeed considered a big at the time,” lead author Ten
Feizi, whose lab Bezouska was working in when he wrote the 1994 paper, said
in an email to BioTechniques. Feizi is now a professor at Imperial College
London.
Since publication, the paper has been cited 257 times since publication,
according to Thomson Reuters Web of Knowledge. “It is most likely that many
of the citations were by scientists unaware of the irreproducibility. I say
this because I made a point of informing key people in the field about the
problem, and between us when refereeing papers citing the article, we
discouraged citation,” said Feizi.
Two years later, a correction to the paper was published in Nature (3). In
that notice, the researchers said that they were unable to reproduce the
results. As a result, the team was re-evaluating the experimental system,
which was set up in Prague by three of the study authors: Bezouska, Anna
Fiserova, and Miloslav Pospíšil. The team promised to publish the
results of their re-evaluation as soon as possible.
But it wasn’t until just last week, 19 years after the original article was
published, that the paper was finally retracted. And that retraction came
without the signatures of Bezouska, Lawson, Pospíšil (now deceased),
and another author who could not be found.
As it turns out, the retraction only came to pass because first author
Bezouska was found by an ethics committee at Charles University in January
2013 to have “most likely” committed scientific misconduct in the
experiments published in the 1994 Nature paper and in follow-up studies, as
reported by Retraction Watch. As a result, Bezouska was terminated from the
Charles University and the Institute of Microbiology. In their press
releases, the 2 organizations urged his coauthors to review the more than
100 peer-reviewed publications that he had published for invalid results.
Although the irreproducible data from the 1994 paper were mentioned in the
report, the most conclusive evidence of Bezouska’s misconduct came from
Professor Vladimir Kren and three students from his laboratory at the
Institute of Microbiology who, in 2012, suspected someone was manipulating
their NKR-P1 carbohydrate binding experiments. So they installed a camera to
monitor their samples overnight. The camera caught Bezouska red-handed,
showing him manipulating the samples to generate positive results.
“…Bezouska fabricated practically all results dealing with NKR-P1 and CD69
receptors,” said Kren in an email to BioTechniques. CD69 receptors are
another surface protein on NK cells. Bezouska claimed to have identified
high-affinity ligands for CD69 receptors in a 1995 Biochemical and
Biophysical Research Communications paper (4). “At present, we estimate
that there [are] some 20 papers contaminated by this misconduct, and these
papers have to be corrected or retracted.”
Although Feizi wanted to retract the 1994 paper when the correction was
published in 1996, Nature’s editorial policy at the time required that
retractions be accompanied by the signature of all authors, according to an
email that she wrote to Retraction Watch. Feizi, however, did publish two
studies in other journals that re-evaluated the results from the 1994 Nature
paper and the 1995 Biochemical and Biophysical Research Communications
paper, concluding that all those results were irreproducible (5-6).
In addition, Feizi published a correction in 1999 to another paper that she
co-authored with Bezouska in 1994 in the Journal of Biological Chemistry,
also on the binding of carbohydrates to NKR-P1 (7-8).
References
1. Bezouska, K., C.-T. Yuen, J. O/'Brien, R. A. Childs, W. Chai, A. M.
Lawson, K. Drbal, A. Fiserova, M. Pospisil, and T. Feizi. 2013. Retraction:
Oligosaccharide ligands for NKR-p1 protein activate NK cells and
cytotoxicity. Nature advance online publication(July).
2. Bezouska, K., C.-T. Yuen, J. O'Brien, R. A. Childs, W. Chai, A. M. Lawson
, K. Drbal, A. Fiserova, M. Posisil, and T. Feizi. 1994. Oligosaccharide
ligands for NKR-p1 protein activate NK cells and cytotoxicity. Nature 372(
6502):150-157.
3.Bezou[caron]ka, K., C.-T. Yuen, J. O'Brien, R. A. Childs, W. Chai, A. M.
Lawson, K. Drbal, A. Fis[caron]erova, M. PospIs[caron]il, and T. Feizi. 1996
. Oligosaccharide ligands for NKR-p1 protein activate NK cells and
cytotoxicity. Nature 380(6574):559.
4. Bezouska, K., A. Nepovím, O. Horváth, M. Pospísil, J. Hamann, and T.
Feizi. 1995. CD 69 antigen of human lymphocytes is a calcium-dependent
carbohydrate-binding protein. Biochemical and biophysical research
communications 208(1):68-74.
5. Kogelberg, H., A. M. Lawson, F. W. Muskett, R. A. Carruthers, and T.
Feizi. 2000. Expression in escherichia coli, folding in vitro, and
characterization of the carbohydrate recognition domain of the natural
killer cell receptor NKR-P1A. Protein Expression and Purification 20(1):10-
20.
6. Childs, R. A., C. Galustian, A. M. Lawson, G. Dougan, K. Benwell, G.
Frankel, and T. Feizi. 1999. Recombinant soluble human CD69 dimer produced
in escherichia coli: Reevaluation of saccharide binding. Biochemical and
Biophysical Research Communications 266(1):19-23.
7. Kogelberg, H., E. Montero, S. Bay, A. M. Lawson, and T. Feizi. 1999. Re-
evaluation of monosaccharide binding property of recombinant soluble
carbohydrate recognition domain of the natural killer cell receptor NKR-P1A.
Journal of Biological Chemistry 274(42):30335.
8. Bezouska, K., G. Vlahas, O. Horváth, G. Jinochová, A. Fiserová, R.
Giorda, W. H. Chambers, T. Feizi, and M. Pospísil. 1994. Rat natural killer
cell antigen, NKR-p1, related to c-type animal lectins is a carbohydrate-
binding protein. Journal of Biological Chemistry 269(24):16945-16952.
y*e
4 楼
已经很不错了吧，如果你是作polymer的话
何时才能高点啊
【在 c*******r 的大作中提到】
: 何时才能高点啊
何时才能高点啊
【在 c*******r 的大作中提到】
: 何时才能高点啊
g*e
5 楼
同问，谢谢！
a*k
6 楼
1994.。。这是在挖坑么？
most
have
【在 n*********m 的大作中提到】
: Immunology Retraction Exposes Misconduct
: 07/10/2013 Andrew S. Wiecek
: Nature retracts a 1994 paper after the first author was found to have "most
: likely" committed research misconduct. Learn more...
: Last week, Nature retracted a highly-cited paper published in 1994 because
: the authors were unable to reproduce the results, according to the
: retraction notice (1). But the real reason behind the retraction might have
: more to do with the first author’s scientific misconduct.
: Nature has retracted a 1994 paper because first author Karel Bezouska was
: found to have "most likely" committed research misconduct. Source: https://
most
have
【在 n*********m 的大作中提到】
: Immunology Retraction Exposes Misconduct
: 07/10/2013 Andrew S. Wiecek
: Nature retracts a 1994 paper after the first author was found to have "most
: likely" committed research misconduct. Learn more...
: Last week, Nature retracted a highly-cited paper published in 1994 because
: the authors were unable to reproduce the results, according to the
: retraction notice (1). But the real reason behind the retraction might have
: more to do with the first author’s scientific misconduct.
: Nature has retracted a 1994 paper because first author Karel Bezouska was
: found to have "most likely" committed research misconduct. Source: https://
b*y
7 楼
BSO啊。
【在 c*******r 的大作中提到】
: 何时才能高点啊
【在 c*******r 的大作中提到】
: 何时才能高点啊
d*y
8 楼
拿你的140申请3年
p*x
9 楼
BSO
bs
bs
g*n
10 楼
1.换了雇主，旧140一样保3年
2.旧H1B可以revoke，旧140没法revoke
2.旧H1B可以revoke，旧140没法revoke
A*d
11 楼
BSO
d*n
12 楼
问了公司的律师。新的h1-b是三年的。这样我就无所谓了。绿卡三年内也排到了。谢谢
各位的回答。
各位的回答。
b*r
13 楼
老子做了这么多年一共才40多，不过我EB1A一次就过啦，哈哈哈，气死楼主
相关阅读
实验照片：谁见过这些亚细胞结构？施一公再一次渔翁得利！发包子，求一篇paper，谢谢！求建议：industry面试talk要注意什么three color confocal?求教：哪个inducible lentiviral shRNA system 比较好？很多大牛也经常灌plos oneE coli在4°C PBS里能happy多久？请问哪个公司做microarray的whole genome expression profiling比较好你们有人去看PhD comics 拍的那个PhD movie了么小时候要做了多少坏事，长大了才和生物结缘啊？？ (转载)我发现我就是一机器Lipidomics 和 metabolomics 是好坑吗？ (转载)又来麻烦大家来。。。。有人参加明年的EB，San Diego 会议么？RNA seq 的样品制备问题第一批青千名单算是正式出来了2012年06月毕业的国内土博什么时候开始联系PostDoc比较好？国内公司经历对以后在美国找公司工作有没有帮助？mammalian cell genomic DNA extraction