把分化的血细胞重编程到造血干细胞# Biology - 生物学
G*G
1 楼
请问大家job talk的时候，是站在哪个位置？
1）手握鼠标，站在台式机的显示器前面
2）手难laser笔，站在教室前台，面对观众，手握laser笔，对着投影幕。
1）手握鼠标，站在台式机的显示器前面
2）手难laser笔，站在教室前台，面对观众，手握laser笔，对着投影幕。
f*g
2 楼
Reprogramming committed murine blood cells to induced hematopoietic stem
cells with defined factors.
Cell,2014
最新一期的Cell发表。
在分化血细胞中高表达6个基因Run1t1, Hlf, Lmo2, Prdm5, Pbx1, and Zfp37 能够重
编程 成造血干细胞。
很有意思，觉得比iPS还要有意思。
我个人认为在各个器官内，可能都存在这种重编程的机制，都有自己的一套转录因子能
够把分化的细胞重编程到祖细胞阶段。
cells with defined factors.
Cell,2014
最新一期的Cell发表。
在分化血细胞中高表达6个基因Run1t1, Hlf, Lmo2, Prdm5, Pbx1, and Zfp37 能够重
编程 成造血干细胞。
很有意思，觉得比iPS还要有意思。
我个人认为在各个器官内，可能都存在这种重编程的机制，都有自己的一套转录因子能
够把分化的细胞重编程到祖细胞阶段。
q*7
3 楼
必须 2）
目光要对着听众，基本不要看屏幕。可以瞟一眼屏幕，不要按错页了:)
目光要对着听众，基本不要看屏幕。可以瞟一眼屏幕，不要按错页了:)
f*g
4 楼
这么有意思的文章没有人感兴趣吗？
难道没有做造血的吗？
这篇文章要是和yamanaka同年发，就厉害了
难道没有做造血的吗？
这篇文章要是和yamanaka同年发，就厉害了
j*i
5 楼
这是可以预期的吧。前一阵不是国内单位有发直接编程到肝细胞。
f*g
6 楼
能给个文章链接吗？
吧什么细胞重编程到肝细胞，用的是yamanaka因子了吗？
【在 j******i 的大作中提到】
: 这是可以预期的吧。前一阵不是国内单位有发直接编程到肝细胞。
吧什么细胞重编程到肝细胞，用的是yamanaka因子了吗？
【在 j******i 的大作中提到】
: 这是可以预期的吧。前一阵不是国内单位有发直接编程到肝细胞。
j*i
7 楼
http://www.cell.com/cell-stem-cell/abstract/S1934-5909(13)00275
【在 f******g 的大作中提到】
: 能给个文章链接吗？
: 吧什么细胞重编程到肝细胞，用的是yamanaka因子了吗？
【在 f******g 的大作中提到】
: 能给个文章链接吗？
: 吧什么细胞重编程到肝细胞，用的是yamanaka因子了吗？
f*g
8 楼
谢谢。
【在 j******i 的大作中提到】
: http://www.cell.com/cell-stem-cell/abstract/S1934-5909(13)00275
【在 j******i 的大作中提到】
: http://www.cell.com/cell-stem-cell/abstract/S1934-5909(13)00275
D*a
9 楼
不是有fibroblast直接编程到neuron了么
f*g
10 楼
能发个link吗？
谢谢。
【在 D*a 的大作中提到】
: 不是有fibroblast直接编程到neuron了么
谢谢。
【在 D*a 的大作中提到】
: 不是有fibroblast直接编程到neuron了么
o*a
11 楼
this one may be not as important as ones that overcome histo-incompatibility
.
hematopoietic stem cells are widely available in bone marrow. why bothers to
make artificial ones ?
【在 f******g 的大作中提到】
: Reprogramming committed murine blood cells to induced hematopoietic stem
: cells with defined factors.
: Cell,2014
: 最新一期的Cell发表。
: 在分化血细胞中高表达6个基因Run1t1, Hlf, Lmo2, Prdm5, Pbx1, and Zfp37 能够重
: 编程 成造血干细胞。
: 很有意思，觉得比iPS还要有意思。
: 我个人认为在各个器官内，可能都存在这种重编程的机制，都有自己的一套转录因子能
: 够把分化的细胞重编程到祖细胞阶段。
.
hematopoietic stem cells are widely available in bone marrow. why bothers to
make artificial ones ?
【在 f******g 的大作中提到】
: Reprogramming committed murine blood cells to induced hematopoietic stem
: cells with defined factors.
: Cell,2014
: 最新一期的Cell发表。
: 在分化血细胞中高表达6个基因Run1t1, Hlf, Lmo2, Prdm5, Pbx1, and Zfp37 能够重
: 编程 成造血干细胞。
: 很有意思，觉得比iPS还要有意思。
: 我个人认为在各个器官内，可能都存在这种重编程的机制，都有自己的一套转录因子能
: 够把分化的细胞重编程到祖细胞阶段。
s*r
12 楼
灌水骗funding
没人关心实用价值
incompatibility
to
【在 o*******a 的大作中提到】
: this one may be not as important as ones that overcome histo-incompatibility
: .
: hematopoietic stem cells are widely available in bone marrow. why bothers to
: make artificial ones ?
没人关心实用价值
incompatibility
to
【在 o*******a 的大作中提到】
: this one may be not as important as ones that overcome histo-incompatibility
: .
: hematopoietic stem cells are widely available in bone marrow. why bothers to
: make artificial ones ?
s*m
13 楼
现在有多少人注册捐骨髓啊？
incompatibility
to
【在 o*******a 的大作中提到】
: this one may be not as important as ones that overcome histo-incompatibility
: .
: hematopoietic stem cells are widely available in bone marrow. why bothers to
: make artificial ones ?
incompatibility
to
【在 o*******a 的大作中提到】
: this one may be not as important as ones that overcome histo-incompatibility
: .
: hematopoietic stem cells are widely available in bone marrow. why bothers to
: make artificial ones ?
X*n
14 楼
尼玛，用一堆ongogene，谁敢用？
f*g
15 楼
在你的眼里，什么样的才不是骗funding?
【在 s******r 的大作中提到】
: 灌水骗funding
: 没人关心实用价值
:
: incompatibility
: to
【在 s******r 的大作中提到】
: 灌水骗funding
: 没人关心实用价值
:
: incompatibility
: to
f*g
16 楼
it is just beginning, more studies will follow it.
【在 X******n 的大作中提到】
: 尼玛，用一堆ongogene，谁敢用？
【在 X******n 的大作中提到】
: 尼玛，用一堆ongogene，谁敢用？
f*g
17 楼
are you talking about this paper:
Identification of a colonial chordate histocompatibility gene.
Science. 2013
or something else?
incompatibility
to
【在 o*******a 的大作中提到】
: this one may be not as important as ones that overcome histo-incompatibility
: .
: hematopoietic stem cells are widely available in bone marrow. why bothers to
: make artificial ones ?
Identification of a colonial chordate histocompatibility gene.
Science. 2013
or something else?
incompatibility
to
【在 o*******a 的大作中提到】
: this one may be not as important as ones that overcome histo-incompatibility
: .
: hematopoietic stem cells are widely available in bone marrow. why bothers to
: make artificial ones ?
X*n
18 楼
就怕不加个oncogene，就不能有这个效果。但这个方法对研究致癌机理应该有借鉴作用
。同期的另一篇Glioblastoma的文章我觉得也很有意思。
http://www.cell.com/cell/abstract/S0092-8674(14)00229-3
【在 f******g 的大作中提到】
: it is just beginning, more studies will follow it.
。同期的另一篇Glioblastoma的文章我觉得也很有意思。
http://www.cell.com/cell/abstract/S0092-8674(14)00229-3
【在 f******g 的大作中提到】
: it is just beginning, more studies will follow it.
f*g
19 楼
They did serial transplantation assay and induced HSCs did not transformed
into tumor cells.
did it mean that these induced HSCs are healthy?
【在 X******n 的大作中提到】
: 尼玛，用一堆ongogene，谁敢用？
into tumor cells.
did it mean that these induced HSCs are healthy?
【在 X******n 的大作中提到】
: 尼玛，用一堆ongogene，谁敢用？
f*g
20 楼
其实，oncogene只是个定义，在基因芯片或者连锁分析发现一些基因在肿瘤中上调，然
后做了一些体外实验，就说这个基因是oncogene,
具体这些oncogene在体内的功能还需要进一步研究，knockin小鼠是不是会产生肿瘤，
如果是胚胎至死的，inducible knockin mouse是不是会引起肿瘤。
如果能引起肿瘤的话，才能说这个基因是oncogene.
【在 X******n 的大作中提到】
: 就怕不加个oncogene，就不能有这个效果。但这个方法对研究致癌机理应该有借鉴作用
: 。同期的另一篇Glioblastoma的文章我觉得也很有意思。
: http://www.cell.com/cell/abstract/S0092-8674(14)00229-3
后做了一些体外实验，就说这个基因是oncogene,
具体这些oncogene在体内的功能还需要进一步研究，knockin小鼠是不是会产生肿瘤，
如果是胚胎至死的，inducible knockin mouse是不是会引起肿瘤。
如果能引起肿瘤的话，才能说这个基因是oncogene.
【在 X******n 的大作中提到】
: 就怕不加个oncogene，就不能有这个效果。但这个方法对研究致癌机理应该有借鉴作用
: 。同期的另一篇Glioblastoma的文章我觉得也很有意思。
: http://www.cell.com/cell/abstract/S0092-8674(14)00229-3
f*g
21 楼
把我以前的贴子顶上来，响应号召
f*g
22 楼
最近一篇nature文章也发表了同样的结果，把人的血液分化细胞重编成到HSC
D*a
23 楼
回答我上次没答的问题，响应号召~
http://www.nature.com/nature/journal/v463/n7284/full/nature0879
【在 f******g 的大作中提到】
: 能发个link吗？
: 谢谢。
http://www.nature.com/nature/journal/v463/n7284/full/nature0879
【在 f******g 的大作中提到】
: 能发个link吗？
: 谢谢。
f*g
24 楼
Reprogramming human endothelial cells to haematopoietic cells requires
vascular induction
Nature
Generating engraftable human haematopoietic cells from autologous tissues is
a potential route to new therapies for blood diseases. However, directed
differentiation of pluripotent stem cells yields haematopoietic cells that
engraft poorly. Here, we have devised a method to phenocopy the vascular-
niche microenvironment of haemogenic cells, thereby enabling reprogramming
of human endothelial cells into engraftable haematopoietic cells without
transition through a pluripotent intermediate. Highly purified non-
haemogenic human umbilical vein endothelial cells or adult dermal
microvascular endothelial cells were transduced with the transcription
factors FOSB, GFI1, RUNX1 and SPI1 (hereafter referred to as FGRS), and then
propagated on serum-free instructive vascular niche monolayers to induce
outgrowth of haematopoietic colonies containing cells with functional and
immunophenotypic features of multipotent progenitor cells (MPPs). These
endothelial cells that have been reprogrammed into human MPPs (rEC-hMPPs)
acquire colony-forming-cell potential and durably engraft into immune-
deficient mice after primary and secondary transplantation, producing long-
term rEC-hMPP-derived myeloid (granulocytic/monocytic, erythroid,
megakaryocytic) and lymphoid (natural killer and B cell) progenies.
Conditional expression of FGRS transgenes, combined with vascular induction,
activates endogenous FGRS genes, endowing rEC-hMPPs with a transcriptional
and functional profile similar to that of self-renewing MPPs. Our approach
underscores the role of inductive cues from the vascular niche in
coordinating and sustaining haematopoietic specification and may prove
useful for engineering autologous haematopoietic grafts to treat inherited
and acquired blood disorders.
vascular induction
Nature
Generating engraftable human haematopoietic cells from autologous tissues is
a potential route to new therapies for blood diseases. However, directed
differentiation of pluripotent stem cells yields haematopoietic cells that
engraft poorly. Here, we have devised a method to phenocopy the vascular-
niche microenvironment of haemogenic cells, thereby enabling reprogramming
of human endothelial cells into engraftable haematopoietic cells without
transition through a pluripotent intermediate. Highly purified non-
haemogenic human umbilical vein endothelial cells or adult dermal
microvascular endothelial cells were transduced with the transcription
factors FOSB, GFI1, RUNX1 and SPI1 (hereafter referred to as FGRS), and then
propagated on serum-free instructive vascular niche monolayers to induce
outgrowth of haematopoietic colonies containing cells with functional and
immunophenotypic features of multipotent progenitor cells (MPPs). These
endothelial cells that have been reprogrammed into human MPPs (rEC-hMPPs)
acquire colony-forming-cell potential and durably engraft into immune-
deficient mice after primary and secondary transplantation, producing long-
term rEC-hMPP-derived myeloid (granulocytic/monocytic, erythroid,
megakaryocytic) and lymphoid (natural killer and B cell) progenies.
Conditional expression of FGRS transgenes, combined with vascular induction,
activates endogenous FGRS genes, endowing rEC-hMPPs with a transcriptional
and functional profile similar to that of self-renewing MPPs. Our approach
underscores the role of inductive cues from the vascular niche in
coordinating and sustaining haematopoietic specification and may prove
useful for engineering autologous haematopoietic grafts to treat inherited
and acquired blood disorders.
B*l
25 楼
Re
Yawnnnnn
【在 s******r 的大作中提到】
: 灌水骗funding
: 没人关心实用价值
:
: incompatibility
: to
Yawnnnnn
【在 s******r 的大作中提到】
: 灌水骗funding
: 没人关心实用价值
:
: incompatibility
: to
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