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求小鼠细胞中WB检测GFP 表达的经验
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求小鼠细胞中WB检测GFP 表达的经验# Biology - 生物学
e*n
1
Sorry I cannot type Chinese now.
I submitted EB-1A in Aug. 2010.
My lawyer filed PP for me on 01/31, but it seems the mail is lost because no
LUD and check is also not cashed. So I plan to file again by myself.
Several queations on I-907, hope someone can help me out.
1. In part 1, You (the person submitting this request):
Should I check the 3rd item: Are the applicant who is filing or has filed an
application eligible for Premium Processing?
2. In part 2, block 3, should I fill 'EB1-A'?
3. In part 2, block 4 and 5, should I just fill my name but not includes my
wife's name?
Many thanks.
avatar
x*3
2
现在正在做WB 检测GFP-Tagged的目的蛋白的表达,尝试了6种GFP 抗体,发现在人的细
胞中都能够检测到GFP 表达,但是在鼠的细胞中只有ABCAM的AB13970检测到GFP 表达(
但有其他一堆杂带),其他的抗体都能看到一堆bands,唯独没有GFP 的bands,主要是
砸带太多,曝光不能爆太久。
有大神能帮忙介绍一下在小鼠细胞中WB检测GFP 表达的经验吗?
不能用目的蛋白的抗体因为做的目的蛋白的truncated protein。
那个abcam的ab13970是鸡抗体,不知道怎么样去优化?(4%BSA block, 400mA/2hrs
transfer)
非常感谢!
avatar
j*u
3
顶一下,同问

no
an
my

【在 e*******n 的大作中提到】
: Sorry I cannot type Chinese now.
: I submitted EB-1A in Aug. 2010.
: My lawyer filed PP for me on 01/31, but it seems the mail is lost because no
: LUD and check is also not cashed. So I plan to file again by myself.
: Several queations on I-907, hope someone can help me out.
: 1. In part 1, You (the person submitting this request):
: Should I check the 3rd item: Are the applicant who is filing or has filed an
: application eligible for Premium Processing?
: 2. In part 2, block 3, should I fill 'EB1-A'?
: 3. In part 2, block 4 and 5, should I just fill my name but not includes my

avatar
c*b
4
是不是用了小鼠做的GFP抗体?换个在兔子里做的GFP抗体试试?

【在 x******3 的大作中提到】
: 现在正在做WB 检测GFP-Tagged的目的蛋白的表达,尝试了6种GFP 抗体,发现在人的细
: 胞中都能够检测到GFP 表达,但是在鼠的细胞中只有ABCAM的AB13970检测到GFP 表达(
: 但有其他一堆杂带),其他的抗体都能看到一堆bands,唯独没有GFP 的bands,主要是
: 砸带太多,曝光不能爆太久。
: 有大神能帮忙介绍一下在小鼠细胞中WB检测GFP 表达的经验吗?
: 不能用目的蛋白的抗体因为做的目的蛋白的truncated protein。
: 那个abcam的ab13970是鸡抗体,不知道怎么样去优化?(4%BSA block, 400mA/2hrs
: transfer)
: 非常感谢!

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